Ontology highlight
ABSTRACT:
SUBMITTER: Uhm H
PROVIDER: S-EPMC5777041 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20171226 2
Because RNAs fold as they are being synthesized, their transcription rate can affect their folding. Here, we report the results of single-molecule fluorescence studies that characterize the ligand-dependent cotranscriptional folding of the <i>Escherichia coli thiM</i> riboswitch that regulates translation. We found that the riboswitch aptamer folds into the "off" conformation independent of its ligand, but switches to the "on" conformation during transcriptional pausing near the translational st ...[more]