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Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery.

ABSTRACT: In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of 'gain-of-function' and 'loss-of-function' blastocyst manipulations can be evaluated with in vitro outgrowth assays or following transfer to pseudopregnant rats.

SUBMITTER: Chakraborty D 

PROVIDER: S-EPMC5777579 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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<i>Ex vivo</i> Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery.

Chakraborty Damayanti D   Muto Masanaga M   Soares Michael J MJ  

Bio-protocol 20171201 24

In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of 'gain-of-function' and 'loss-of-function' blastocyst manipulations can be evaluated with <i>in vitro</i> outgrowth assays or following transfer to pseudopregnant rats. ...[more]

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