Unknown

Dataset Information

0

Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-?B signaling.

ABSTRACT: In this study, we investigated the roles of reactive oxygen species (ROS) and nuclear factor-?B (NF-?B) signaling in sodium fluoride-induced DNA damage and apoptosis in mouse splenocytes. Intragastric administration of 12, 24 or 48 mg/kg sodium fluoride resulted in a time- and dose-dependent increase in DNA fragmentation and apoptosis in mouse splenocytes on days 21 and 42. High ROS levels correlated with increased levels of phosphorylated I?B kinase and NF-?B p65 and decreased levels of inhibitory kappa B protein in splenocytes from mice treated with sodium fluoride. Moreover, splenocytes from sodium fluoride-treated mice showed high expression of pro-apoptotic proteins, including Bim, Bax, Bak, caspase-3 and poly ADP-ribose polymerase, and low expression of the anti-apoptotic proteins BcL-2 and BcL-xL. These results show that sodium fluoride induces apoptosis in mouse splenocytes by enhancing ROS-dependent NF-?B signaling.

SUBMITTER: Deng H 

PROVIDER: S-EPMC5777703 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-κB signaling.

Deng Huidan H   Kuang Ping P   Cui Hengmin H   Luo Qin Q   Liu Huan H   Lu Yujiao Y   Fang Jing J   Zuo Zhicai Z   Deng Junliang J   Li Yinglun Y   Wang Xun X   Zhao Ling L  

Oncotarget 20171201 70

In this study, we investigated the roles of reactive oxygen species (ROS) and nuclear factor-κB (NF-κB) signaling in sodium fluoride-induced DNA damage and apoptosis in mouse splenocytes. Intragastric administration of 12, 24 or 48 mg/kg sodium fluoride resulted in a time- and dose-dependent increase in DNA fragmentation and apoptosis in mouse splenocytes on days 21 and 42. High ROS levels correlated with increased levels of phosphorylated IκB kinase and NF-κB p65 and decreased levels of inhibit  ...[more]

Similar Datasets

Unknown Sodium fluoride induces renal inflammatory responses by activating NF-κB signaling pathway and reducing anti-inflammatory cytokine expression in mice.
| S-EPMC5655190 | biostudies-literature
Unknown Sodium fluoride induces apoptosis in mouse embryonic stem cells through ROS-dependent and caspase- and JNK-mediated pathways.
| S-EPMC3299821 | biostudies-literature
Unknown Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF-κB signaling pathways.
| S-EPMC8855797 | biostudies-literature
Unknown Human Norovirus Induces Aquaporin 1 Production by Activating NF-κB Signaling Pathway.
| S-EPMC9028284 | biostudies-literature
Unknown PLCA8 suppresses breast cancer apoptosis by activating the PI3k/AKT/NF-κB pathway.
| S-EPMC6787500 | biostudies-literature
Unknown The Nedd8-activating enzyme inhibitor MLN4924 thwarts microenvironment-driven NF-κB activation and induces apoptosis in chronic lymphocytic leukemia B cells.
| S-EPMC3960291 | biostudies-literature
Unknown Hyperoside Induces Breast Cancer Cells Apoptosis via ROS-Mediated NF-?B Signaling Pathway.
| S-EPMC6981893 | biostudies-literature
Unknown Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB.
| S-EPMC4396137 | biostudies-literature
Unknown Tripartite motif-containing 25 facilitates immunosuppression and inhibits apoptosis of glioma via activating NF-κB.
| S-EPMC9554170 | biostudies-literature
Unknown Inhibition of NF-κB by deoxycholic acid induces miR-21/PDCD4-dependent hepatocellular apoptosis.
| S-EPMC4664913 | biostudies-literature