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Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-?B signaling.

ABSTRACT: In this study, we investigated the roles of reactive oxygen species (ROS) and nuclear factor-?B (NF-?B) signaling in sodium fluoride-induced DNA damage and apoptosis in mouse splenocytes. Intragastric administration of 12, 24 or 48 mg/kg sodium fluoride resulted in a time- and dose-dependent increase in DNA fragmentation and apoptosis in mouse splenocytes on days 21 and 42. High ROS levels correlated with increased levels of phosphorylated I?B kinase and NF-?B p65 and decreased levels of inhibitory kappa B protein in splenocytes from mice treated with sodium fluoride. Moreover, splenocytes from sodium fluoride-treated mice showed high expression of pro-apoptotic proteins, including Bim, Bax, Bak, caspase-3 and poly ADP-ribose polymerase, and low expression of the anti-apoptotic proteins BcL-2 and BcL-xL. These results show that sodium fluoride induces apoptosis in mouse splenocytes by enhancing ROS-dependent NF-?B signaling.

SUBMITTER: Deng H

PROVIDER: S-EPMC5777703 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-κB signaling.

Deng Huidan H Kuang Ping P Cui Hengmin H Luo Qin Q Liu Huan H Lu Yujiao Y Fang Jing J Zuo Zhicai Z Deng Junliang J Li Yinglun Y Wang Xun X Zhao Ling L

Oncotarget 20171201 70

In this study, we investigated the roles of reactive oxygen species (ROS) and nuclear factor-κB (NF-κB) signaling in sodium fluoride-induced DNA damage and apoptosis in mouse splenocytes. Intragastric administration of 12, 24 or 48 mg/kg sodium fluoride resulted in a time- and dose-dependent increase in DNA fragmentation and apoptosis in mouse splenocytes on days 21 and 42. High ROS levels correlated with increased levels of phosphorylated IκB kinase and NF-κB p65 and decreased levels of inhibit ...[more]

PMID: 29383091

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Dataset Information

Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-?B signaling.

Publications

Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-κB signaling.

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