Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids.
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ABSTRACT:
SUBMITTER: Exterkate M
PROVIDER: S-EPMC5778391 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids.
Exterkate Marten M Caforio Antonella A Stuart Marc C A MCA Driessen Arnold J M AJM
ACS synthetic biology 20171002 1
One of the key aspects that defines a cell as a living entity is its ability to self-reproduce. In this process, membrane biogenesis is an essential element. Here, we developed an in vitro phospholipid biosynthesis pathway based on a cascade of eight enzymes, starting from simple fatty acid building blocks and glycerol 3-phosphate. The reconstituted system yields multiple phospholipid species that vary in acyl-chain and polar headgroup compositions. Due to the high fidelity and versatility, comp ...[more]
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