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AZGP1 inhibits soft tissue sarcoma cells invasion and migration.


ABSTRACT: One of the major challenges in soft tissue sarcomas is to identify factors that predict metastasis. AZGP1 is a potential biomarker of cancer progression, but its value in soft tissue sarcomas remains unknown. The aim of this study is to determine the expression level of AZGP1 in soft tissue sarcomas, and to analyze its influence on tumor progression.AZGP1 immunohistochemistry (IHC) and RT-PCR were performed in 86 patients with soft tissue sarcomas. The relationships between AZGP1 levels and clinicopathologic features were analyzed. In vitro experiments were performed using fibrosarcoma (HT1080), rhabdomyosarcoma (RD) and synovial sarcoma (SW982) cell lines to corroborate our findings. We used lentiviral over-expression and knockdown assays to examine how changes of AZGP1 expressions might affect cellular migration and invasion.The quantitative RT-PCR results showed that AZGP1 expression was negatively correlated with metastasis and overall survival in soft tissue sarcomas (p?

SUBMITTER: Liu J 

PROVIDER: S-EPMC5778744 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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AZGP1 inhibits soft tissue sarcoma cells invasion and migration.

Liu Jiayong J   Han Haibo H   Fan Zhengfu Z   El Beaino Marc M   Fang Zhiwei Z   Li Shu S   Ji Jiafu J  

BMC cancer 20180122 1


<h4>Background</h4>One of the major challenges in soft tissue sarcomas is to identify factors that predict metastasis. AZGP1 is a potential biomarker of cancer progression, but its value in soft tissue sarcomas remains unknown. The aim of this study is to determine the expression level of AZGP1 in soft tissue sarcomas, and to analyze its influence on tumor progression.<h4>Methods</h4>AZGP1 immunohistochemistry (IHC) and RT-PCR were performed in 86 patients with soft tissue sarcomas. The relation  ...[more]

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