Two-step interphase microtubule disassembly aids spindle morphogenesis.

ABSTRACT:

Background

Entry into mitosis triggers profound changes in cell shape and cytoskeletal organisation. Here, by studying microtubule remodelling in human flat mitotic cells, we identify a two-step process of interphase microtubule disassembly.

Results

First, a microtubule-stabilising protein, Ensconsin/MAP7, is inactivated in prophase as a consequence of its phosphorylation downstream of Cdk1/cyclin B. This leads to a reduction in interphase microtubule stability that may help to fuel the growth of centrosomally nucleated microtubules. The peripheral interphase microtubules that remain are then rapidly lost as the concentration of tubulin heterodimers falls following dissolution of the nuclear compartment boundary. Finally, we show that a failure to destabilise microtubules in

SUBMITTER: Mchedlishvili N 

PROVIDER: S-EPMC5778756 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature