Metabolic engineering of Escherichia coli for the biosynthesis of 2-pyrrolidone.
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SUBMITTER: Zhang J
PROVIDER: S-EPMC5779725 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
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Metabolic engineering of <i>Escherichia coli</i> for the biosynthesis of 2-pyrrolidone.
Zhang Jingwei J Kao Emily E Wang George G Baidoo Edward E K EEK Chen Matthew M Keasling Jay D JD
Metabolic engineering communications 20151110
2-Pyrrolidone is a valuable bulk chemical with myriad applications as a solvent, polymer precursor and active pharmaceutical intermediate. A novel 2-pyrrolidone synthase, ORF27, from <i>Streptomyces aizunensis</i> was identified to catalyze the ring closing dehydration of γ-aminobutyrate. ORF27's tendency to aggregate was resolved by expression at low temperature and fusion to the maltose binding protein (MBP). Recombinant <i>Escherichia coli</i> was metabolically engineered for the production o ...[more]
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