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Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains.


ABSTRACT: The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. pylori exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference of spatial and temporal changes of metabolic activities during biofilm formation. Our study seeks to examine the differences in metabolome of low and high biofilm-formers using the metabolomic approach. Eight H. pylori clinical strains with different biofilm-forming-capability were chosen for metabolomic analysis. Bacterial metabolites were extracted using Bligh and Dyer method and analyzed by Liquid Chromatography/Quadrupole Time-of-Flight mass spectrometry. The data was processed and analyzed using the MassHunter Qualitative Analysis and the Mass Profiler Professional programs. Based on global metabolomic profiles, low and high biofilm-formers presented as two distinctly different groups. Interestingly, low-biofilm-formers produced more metabolites than high-biofilm-formers. Further analysis was performed to identify metabolites that differed significantly (p-value?

SUBMITTER: Wong EHJ 

PROVIDER: S-EPMC5780479 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains.

Wong Eric Hong Jian EHJ   Ng Chow Goon CG   Goh Khean Lee KL   Vadivelu Jamuna J   Ho Bow B   Loke Mun Fai MF  

Scientific reports 20180123 1


The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. pylori exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference of spatial and temporal changes of metabolic activities during biofilm formation. Our study seeks to examine the differences in metabolome of low and high biofilm-formers using the metabolomic approach. Eight H. pylori clinical strains with different bio  ...[more]

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