Cost-effective downstream processing of recombinantly produced pexiganan peptide and its antimicrobial activity.

ABSTRACT: Antimicrobial peptides (AMPs) have significant potential as alternatives to classical antibiotics. However, AMPs are currently prepared using processes which are often laborious, expensive and of low-yield, thus hindering their research and application. Large-scale methods for production of AMPs using a cost-effective approach is urgently required. In this study, we report a scalable, chromatography-free downstream processing method for producing an antimicrobial peptide, pexiganan, using recombinant Escherichia coli (E. coli). The four helix bundle structure of the unique carrier protein DAMP4 was used to facilitate a simple and cheap purification process based on a selective thermochemical precipitation. Highly pure fusion protein DAMP4_var-pexiganan was obtained at high yield

SUBMITTER: Sun B 

PROVIDER: S-EPMC5783979 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature