Ontology highlight
ABSTRACT:
SUBMITTER: Hu X
PROVIDER: S-EPMC5785341 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Hu Xixun X Meng Xiangbing X Liu Qing Q Li Jiayang J Wang Kejian K
Plant biotechnology journal 20170805 1
Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) is a revolutionary technology that enables efficient genomic modification in many organisms. Currently, the wide use of Streptococcus pyogenes Cas9 (SpCas9) primarily recognizes sites harbouring a canonical NGG protospacer adjacent motif (PAM). The newly developed VQR (D1135V/R1335Q/T1337R) variant of Cas9 has been shown to cleave sites containing NGA PAM in rice, which greatly expanded the range of geno ...[more]