Dataset Information

Blood Glutamate Levels Are Closely Related to Acute Lung Injury and Prognosis after Stroke.

ABSTRACT: Background:Acute lung injury (ALI) is a serious complication of stroke that occurs with a high incidence. Our preclinical results indicated that ALI might be related to blood glutamate levels after brain injury. The purpose of this study was to assess dynamic changes in blood glutamate levels in patients with stroke and to determine the correlation between blood glutamate levels, ALI, and long-term prognosis after stroke. Methods:Venous blood samples were collected from controls and patients with stroke at admission and on the third and seventh day after the onset of stroke. Patients were followed for 3?months. The correlations among blood glutamate levels, severities of stroke and ALI, and long-term outcomes were analyzed, and the predictive values of blood glutamate levels and severity scores for ALI were assessed. Results:In this study, a total of 384 patients with stroke were enrolled, with a median age of 59?years. Patients showed significantly increased blood glutamate levels within 7?days of stroke onset (p?

SUBMITTER: Bai W

PROVIDER: S-EPMC5785722 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Blood Glutamate Levels Are Closely Related to Acute Lung Injury and Prognosis after Stroke.

Bai Wei W Li Wei W Ning Ya-Lei YL Li Ping P Zhao Yan Y Yang Nan N Jiang Yu-Lin YL Liang Ze-Ping ZP Jiang Dong-Po DP Wang Ying Y Zhang Meng M Zhou Yuan-Guo YG

Frontiers in neurology 20180119

<h4>Background</h4>Acute lung injury (ALI) is a serious complication of stroke that occurs with a high incidence. Our preclinical results indicated that ALI might be related to blood glutamate levels after brain injury. The purpose of this study was to assess dynamic changes in blood glutamate levels in patients with stroke and to determine the correlation between blood glutamate levels, ALI, and long-term prognosis after stroke.<h4>Methods</h4>Venous blood samples were collected from controls a ...[more]

PMID: 29403427

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Project description:Heat stroke can induce a systemic inflammatory response, which may lead to multi‑organ dysfunction including acute kidney injury (AKI) and electrolyte disturbances. To investigate the pathogenesis of heat stroke (HS)‑related AKI, a mouse model of HS was induced by increasing the animal's core temperature to 41˚C. Blood samples obtained from the tail vein were used to measure plasma glucose and creatinine levels. Micro‑positron emission tomography‑computed tomography (micro‑PET/CT), H&E staining and transmission electron microscopy were conducted to examine metabolic and morphological changes in the mouse kidneys. Immunohistochemistry (IHC) and western blot analyses were performed to investigate the expression of apoptosis‑inducing factor mitochondria‑associated 2 (Aifm2), high‑mobility group box 1 (HMGB1) and receptor for advanced glycosylation end products (RAGE). Liquid chromatography‑mass spectrometry analysis was conducted to find differential metabolites and signaling pathways. The HS mouse model was built successfully, with significantly increased creatinine levels detected in the serum of HS mice compared with controls, whereas micro‑PET/CT revealed active metabolism in the whole body of HS mice. H&E and TUNEL staining revealed that the kidneys of HS mice exhibited signs of hemorrhage and apoptosis. IHC and western blotting demonstrated significant upregulation of Aifm2, HMGB1 and RAGE in response to HS. Finally, 136 differential metabolites were screened out, and enrichment of the 'biosynthesis of unsaturated fatty acids' pathway was detected. HS‑associated AKI is the renal manifestation of systemic inflammatory response syndrome, and may be triggered by the HMGB1/RAGE pathway. Metabolomics indicated increased adrenic acid, docosahexaenoic acid and eicosapentaenoic acid may serve as metabolic biomarkers for AKI in HS. The findings suggested that a correlation between the HMGB1/RAGE pathway and biosynthesis of unsaturated fatty acids may contribute to the progression of HS‑related AKI.

Dataset Information

Blood Glutamate Levels Are Closely Related to Acute Lung Injury and Prognosis after Stroke.

Publications

Blood Glutamate Levels Are Closely Related to Acute Lung Injury and Prognosis after Stroke.

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