Dataset Information

Environmental Factors Support the Formation of Specific Bacterial Assemblages on Microplastics.

ABSTRACT: While the global distribution of microplastics (MP) in the marine environment is currently being critically evaluated, the potential role of MP as a vector for distinct microbial assemblages or even pathogenic bacteria is hardly understood. To gain a deeper understanding, we investigated how different in situ conditions contribute to the composition and specificity of MP-associated bacterial communities in relation to communities on natural particles. Polystyrene (PS), polyethylene (PE), and wooden pellets were incubated for 2 weeks along an environmental gradient, ranging from marine (coastal Baltic Sea) to freshwater (waste water treatment plant, WWTP) conditions. The associated assemblages as well as the water communities were investigated applying high-throughput 16S rRNA gene sequencing. Our setup allowed for the first time to determine MP-dependent and -independent assemblage factors as subject to different environmental conditions in one system. Most importantly, plastic-specific assemblages were found to develop solely under certain conditions, such as lower nutrient concentration and higher salinity, while the bacterial genus Erythrobacter, known for the ability to utilize polycyclic aromatic hydrocarbons (PAH), was found specifically on MP across a broader section of the gradient. We discovered no enrichment of potential pathogens on PE or PS; however, the abundant colonization of MP in a WWTP by certain bacteria commonly associated with antibiotic resistance suggests MP as a possible hotspot for horizontal gene transfer. Taken together, our study clarifies that the surrounding environment prevailingly shapes the biofilm communities, but that MP-specific assemblage factors exist. These findings point to the ecological significance of specific MP-promoted bacterial populations in aquatic environments and particularly in plastic accumulation zones.

SUBMITTER: Oberbeckmann S

PROVIDER: S-EPMC5785724 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Environmental Factors Support the Formation of Specific Bacterial Assemblages on Microplastics.

Oberbeckmann Sonja S Kreikemeyer Bernd B Labrenz Matthias M

Frontiers in microbiology 20180119

While the global distribution of microplastics (MP) in the marine environment is currently being critically evaluated, the potential role of MP as a vector for distinct microbial assemblages or even pathogenic bacteria is hardly understood. To gain a deeper understanding, we investigated how different <i>in situ</i> conditions contribute to the composition and specificity of MP-associated bacterial communities in relation to communities on natural particles. Polystyrene (PS), polyethylene (PE), ...[more]

PMID: 29403454

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Project description:Microplastics in aquatic environments provide novel habitats for surface-colonizing microorganisms. Given the continuing debate on whether substrate-specific properties or environmental factors prevail in shaping biofilm assemblages on microplastics, we examined the influence of substrate vs. spatial factors in the development of bacterial assemblages on polyethylene (PE), polystyrene (PS), wood, and seston and in the free-living fraction. Further, the selective colonization of microplastics by potential pathogens was investigated because among the bacterial species found in microplastic-associated biofilms are potentially pathogenic Vibrio spp. Due to their persistence and great dispersal potential, microplastics could act as vectors for these potential pathogens and for biofilm assemblages in general. Incubation experiments with these substrates were conducted for 7 days during a summer cruise along the eastern Baltic Sea coastline in waters covering a salinity gradient of 4.5-9 PSU. Bacterial assemblages were analyzed using 16S rRNA-gene amplicon sequencing, distance-based redundancy analyses, and the linear discriminant analysis effect size method to identify taxa that were significantly more abundant on the plastics. The results showed that the sample type was the most important factor structuring bacterial assemblages overall. Surface properties were less significant in differentiating attached biofilms on PE, PS, and wood; instead, environmental factors, mainly salinity, prevailed. A potential role for inorganic-nutrient limitations in surface-specific attachment was identified as well. Alphaproteobacteria (Sphingomonadaceae, Devosiaceae, and Rhodobacteraceae) and Gammaproteobacteria (Alteromonadaceae and Pseudomonas) were distinctive for the PE- and PS-associated biofilms. Vibrio was more abundant on the PE and PS biofilms than on seston, but its abundances were highest on wood and positively correlated with salinity. These results corroborate earlier findings that microplastics constitute a habitat for biofilm-forming microorganisms distinct from seston, but less from wood. In contrast to earlier reports of low Vibrio numbers on microplastics, these results also suggest that vibrios are early colonizers of surfaces in general. Spatial as well as temporal dynamics should therefore be considered when assessing the potential of microplastics to serve as vectors for bacterial assemblages and putative pathogens, as these parameters are major drivers of biofilm diversity.

Project description:Microplastics, plastic particles less than 5 mm in length, are a ubiquitous pollutant in the environment, but research on freshwater microplastic contamination is lacking. A possible fate of microplastics in freshwater environments is to become entangled or aggregated in biofilms, which are matrices of algae, bacteria, and micro invertebrates that grow on underwater surfaces, following a progression of settling algae, periphyton, and finally invertebrate colonization. This in-situ study at the Oasis Marina at National Harbor in Oxon Hill, Maryland, examined how the taxonomic assemblages of freshwater biofilms in the Potomac River are associated with the number of microplastics aggregated within them. Aluminum discs, acting as artificial substrate for biofilm growth, were deployed at the water's surface and at 2 m depth to survey biofilm assemblage and were sampled monthly from October 2021-October 2022. Microplastic abundances in the water column were measured every 2 weeks over the same period. Spatial and temporal trends in trapped and suspended microplastics, water quality parameters (temperature, dissolved oxygen, pH, salinity, conductivity, turbidity, ammonia, nitrate, and phosphate), and biofilm assemblages were measured and compared to explore factors affecting the abundance of microplastics and their partitioning between the water column and biofilms. Water quality had no measurable impact on microplastic abundance in the water column at either depth, but temperature was negatively correlated to microplastic abundance in biofilms. As the weather warmed and biofilms progressed to invertebrate settling, they tended to contain fewer microplastics. This may have occurred because less biologically rich biofilms, primarily composed of unicellular algal colonies, provide a favorable surface for microplastic deposition. Understanding seasonal changes in biofilm assemblage and microplastic abundance may help track the fate of microplastics in freshwater systems, particularly in their interactions with lower trophic organisms.

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Dataset Information

Environmental Factors Support the Formation of Specific Bacterial Assemblages on Microplastics.

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Environmental Factors Support the Formation of Specific Bacterial Assemblages on Microplastics.

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