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Monoclonal and oligoclonal TCR AV and BV gene usage in CD4⁺ T cells from pigs immunised with C-strain CSFV vaccine.

ABSTRACT: The classical swine fever virus C-strain vaccine (C-strain vaccine) plays a vital role in preventing and controlling the spread of classical swine fever (CSF). However, the protective mechanisms of C-strain vaccine and cellular immunity conferred by T cell receptors (TCRs) are less well defined. We aimed to analyse the association between the complementarity determining region 3 (CDR3) spectratype of ??TCR in CD4⁺ T cells and C-strain vaccine; and to find conserved CDR3 amino acid motifs in specific TCR ?- and ?-chains. We found that the CDR3 spectratype showed dynamic changes correlating with C-strain vaccine immunisation and that TCR AV5S/8-3S/8-4S/14/38 and BV4S/6S/7S/15S/30 gene families showed clonal expansion in immunised pigs. The sequences of CDR3 from these clonally expanded T cells indicated a high frequency of the 'KLX' motif in the TCR ? chain and the 'GGX' motif in ? chain, and J?39, J?43, J?2.5 and J?2.3 genes were also found in high frequency. To the best of our knowledge, this is the first report describing the dynamic changes of ??TCRs and conserved CDR3 amino acid motifs in CD4⁺ T cells from C-strain vaccine-immunised pigs, which will provide a basis for the development of high-efficiency epitope vaccines.

SUBMITTER: Wang C

PROVIDER: S-EPMC5786037 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Monoclonal and oligoclonal TCR AV and BV gene usage in CD4<sup>+</sup> T cells from pigs immunised with C-strain CSFV vaccine.

Wang Chunyan C Li Shoujie S Jia Huaijie H Chen Guohua G Fang Yongxiang Y Zeng Shuang S He Xiaobing X Yao Wenjuan W Jin Qiwang Q Cheng Wenyu W Feng Yuan Y Yin Hong H Jing Zhizhong Z

Scientific reports 20180126 1

The classical swine fever virus C-strain vaccine (C-strain vaccine) plays a vital role in preventing and controlling the spread of classical swine fever (CSF). However, the protective mechanisms of C-strain vaccine and cellular immunity conferred by T cell receptors (TCRs) are less well defined. We aimed to analyse the association between the complementarity determining region 3 (CDR3) spectratype of αβTCR in CD4<sup>+</sup> T cells and C-strain vaccine; and to find conserved CDR3 amino acid mot ...[more]

PMID: 29374266

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Project description:BackgroundThe tau positron emission tomography (PET) ligand 18F-flortaucipir binds to paired helical filaments of tau in aging and Alzheimer's disease (AD), but its utility in detecting tau aggregates in frontotemporal dementia (FTD) is uncertain.MethodsWe performed 18F-flortaucipir imaging in patients with the FTD syndromes (n = 45): nonfluent variant primary progressive aphasia (nfvPPA) (n = 11), corticobasal syndrome (CBS) (n = 10), behavioral variant frontotemporal dementia (bvFTD) (n = 10), semantic variant primary progressive aphasia (svPPA) (n = 2) and FTD associated pathogenic genetic mutations microtubule-associated protein tau (MAPT) (n = 6), chromosome 9 open reading frame 72 (C9ORF72) (n = 5), and progranulin (GRN) (n = 1). All patients underwent MRI and β-amyloid biomarker testing via 11C-PiB or cerebrospinal fluid. 18F-flortaucipir uptake in patients was compared to 53 β-amyloid negative normal controls using voxelwise and pre-specified region of interest approaches.ResultsOn qualitative assessment, patients with nfvPPA showed elevated 18F-flortacupir binding in the left greater than right inferior frontal gyrus. Patients with CBS showed elevated binding in frontal white matter, with higher cortical gray matter uptake in a subset of β-amyloid-positive patients. Five of ten patients with sporadic bvFTD demonstrated increased frontotemporal binding. MAPT mutation carriers had elevated 18F-flortaucipir retention primarily, but not exclusively, in mutations with Alzheimer's-like neurofibrillary tangles. However, tracer retention was also seen in patients with svPPA, and the mutations C9ORF72, GRN predicted to have TDP-43 pathology. Quantitative region-of-interest differences between patients and controls were seen only in inferior frontal gyrus in nfvPPA and left insula and bilateral temporal poles in MAPT carriers. No significant regional differences were found in CBS or sporadic bvFTD. Two patients underwent postmortem neuropathological examination. A patient with C9ORF72, TDP-43-type B pathology, and incidental co-pathology of scattered neurofibrillary tangles in the middle frontal, inferior temporal gyrus showed corresponding mild 18F-flortaucipir retention without additional uptake matching the widespread TDP-43 type B pathology. A patient with sporadic bvFTD demonstrated punctate inferior temporal and hippocampus tracer retention, corresponding to the area of severe argyrophilic grain disease pathology.Conclusions18F-flortaucipir in patients with FTD and predicted tauopathy or TDP-43 pathology demonstrated limited sensitivity and specificity. Further postmortem pathological confirmation and development of FTD tau-specific ligands are needed.

Dataset Information

Monoclonal and oligoclonal TCR AV and BV gene usage in CD4⁺ T cells from pigs immunised with C-strain CSFV vaccine.

Publications

Monoclonal and oligoclonal TCR AV and BV gene usage in CD4<sup>+</sup> T cells from pigs immunised with C-strain CSFV vaccine.

Similar Datasets

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Dataset Information

Monoclonal and oligoclonal TCR AV and BV gene usage in CD4+ T cells from pigs immunised with C-strain CSFV vaccine.

Publications

Monoclonal and oligoclonal TCR AV and BV gene usage in CD4<sup>+</sup> T cells from pigs immunised with C-strain CSFV vaccine.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Monoclonal and oligoclonal TCR AV and BV gene usage in CD4⁺ T cells from pigs immunised with C-strain CSFV vaccine.