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Expression Profiles of the Trehalose-6-Phosphate Synthase Gene Associated With Thermal Stress in Ostrinia furnacalis (Lepidoptera: Crambidae)

ABSTRACT: Abstract Trehalose is the major blood sugar in insects. Physiological significance of this compound has been extensively reported. Trehalose-6-phosphate synthase (TPS) is an important enzyme in the trehalose biosynthesis pathway. Full-length cDNAs of TPS (Of?tps) and its alternative splicing isoform (Of?tps_isoformI) were cloned from the Asian corn borer (ACB), Ostrinia furnacalis (Guenée; Lepidoptera: Crambidae) larvae. The Of?tps and Of?tps_isoformI transcripts were 2913 and 1689 bp long, contained 2529 and 1293 bp open reading frames encoding proteins of 842 and 430 amino acids with a molecular mass of 94.4 and 48.6 kDa, respectively. Transcriptional profiling and response to thermal stress of Of?tps gene were determined by quantitative real-time PCR showing that the Of?tps was predominantly expressed in the larval fat body, significantly enhanced during molting and transformation; and thermal stress also induced Of?tps expression. Gene structure analysis is indicating that one TPS domain and one trehalose-6-phosphate phosphatase (TPP) domain were located at the N- and C-termini of Of????????TPS, respectively, while only the TPS domain was detected in OfTPS_isoformI. Three-dimensional modeling and heterologous expression were developed to predict the putative functions of OfTPS and Of???TPS_isoformI. We infer that the expression of Of?tps gene is thermally induced and might be crucial for larvae survival.

SUBMITTER: Jin T

PROVIDER: S-EPMC5786230 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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