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Application of a ?-3 Desaturase with an Arachidonic Acid Preference to Eicosapentaenoic Acid Production in Mortierella alpina.


ABSTRACT: In the industrial oleaginous fungus Mortierella alpina, the arachidonic acid (AA; C20:4; ?-6) fraction can reach 50% of the total fatty acids (TFAs) in vivo. However, the eicosapentaenoic acid (EPA; C20:5; ?-3) fraction is less than 3% when this fungus is cultivated at a low temperature (12°C). Omega-3 fatty acid desaturase is a key enzyme in ?-3 long-chain polyunsaturated fatty acids biosynthesis pathways. To enhance EPA production, we transformed the ?-3 fatty acid desaturase (PaD17), which exhibits strong ?-17 desaturase activity, into M. alpina, thus increasing the AA to EPA conversion rate to 49.8%. This PaD17-harboring M. alpina reconstruction strain produced 617?mg L-1 of EPA at room temperature in broth medium, this yield was increased to 1.73?g L-1 after culture medium optimization (i.e., about threefold higher than that under original culture conditions), with concomitant respective increases in dry cell weight and TFA content to 16.55 and 6.46?g L-1. These findings suggest a new platform for the future industrial production of EPA.

SUBMITTER: Ge C 

PROVIDER: S-EPMC5786553 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Application of a ω-3 Desaturase with an Arachidonic Acid Preference to Eicosapentaenoic Acid Production in <i>Mortierella alpina</i>.

Ge Chengfeng C   Chen Haiqin H   Mei Tiantian T   Tang Xin X   Chang Lulu L   Gu Zhennan Z   Zhang Hao H   Chen Wei W   Chen Yong Q YQ  

Frontiers in bioengineering and biotechnology 20180122


In the industrial oleaginous fungus <i>Mortierella alpin</i>a, the arachidonic acid (AA; C20:4; ω-6) fraction can reach 50% of the total fatty acids (TFAs) <i>in vivo</i>. However, the eicosapentaenoic acid (EPA; C20:5; ω-3) fraction is less than 3% when this fungus is cultivated at a low temperature (12°C). Omega-3 fatty acid desaturase is a key enzyme in ω-3 long-chain polyunsaturated fatty acids biosynthesis pathways. To enhance EPA production, we transformed the ω-3 fatty acid desaturase (Pa  ...[more]

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