Ontology highlight
ABSTRACT:
SUBMITTER: de Groot R
PROVIDER: S-EPMC5787707 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
de Groot Reinoud R Lüthi Joel J Lindsay Helen H Lindsay Helen H Holtackers René R Pelkmans Lucas L
Molecular systems biology 20180123 1
High-content imaging using automated microscopy and computer vision allows multivariate profiling of single-cell phenotypes. Here, we present methods for the application of the CISPR-Cas9 system in large-scale, image-based, gene perturbation experiments. We show that CRISPR-Cas9-mediated gene perturbation can be achieved in human tissue culture cells in a timeframe that is compatible with image-based phenotyping. We developed a pipeline to construct a large-scale arrayed library of 2,281 sequenc ...[more]