Ontology highlight
ABSTRACT:
SUBMITTER: Chen X
PROVIDER: S-EPMC5788913 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Chen Xiaoxu X Yang Xiaoyu X Shen Yu Y Hou Jin J Bao Xiaoming X
Frontiers in microbiology 20180125
Malonyl-coenzyme A (malonyl-CoA) is a critical precursor for the biosynthesis of a variety of biochemicals. It is synthesized by the catalysis of acetyl-CoA carboxylase (Acc1p), which was demonstrated to be deactivated by the phosphorylation of Snf1 protein kinase in yeast. In this study, we designed a synthetic malonyl-CoA biosensor and used it to screen phosphorylation site mutations of Acc1p in <i>Saccharomyces cerevisiae</i>. Thirteen phosphorylation sites were mutated, and a combination of ...[more]