Ontology highlight
ABSTRACT:
SUBMITTER: Booth WT
PROVIDER: S-EPMC5793033 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
ACS omega 20180122 1
For years, the use of polyhistidine tags (His-tags) has been a staple in the isolation of recombinant proteins in immobilized metal affinity chromatography experiments. Their usage has been widely beneficial in increasing protein purity from crude cell lysates. For some recombinant proteins, a consequence of His-tag addition is that it can affect protein function and stability. Functional proteins are essential in the elucidation of their biological, kinetic, structural, and thermodynamic proper ...[more]