Ontology highlight
ABSTRACT:
SUBMITTER: Chari R
PROVIDER: S-EPMC5793212 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Chari Raj R Yeo Nan Cher NC Chavez Alejandro A Church George M GM
ACS synthetic biology 20170210 5
It has been possible to create tools to predict single guide RNA (sgRNA) activity in the CRISPR/Cas9 system derived from Streptococcus pyogenes due to the large amount of data that has been generated in sgRNA library screens. However, with the discovery of additional CRISPR systems from different bacteria, which show potent activity in eukaryotic cells, the approach of generating large data sets for each of these systems to predict their activity is not tractable. Here, we present a new guide RN ...[more]