Ontology highlight
ABSTRACT:
SUBMITTER: Lerner R
PROVIDER: S-EPMC5794423 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Lerner Raissa R Post Julia M JM Ellis Shane R SR Vos D R Naomi DRN Heeren Ron M A RMA Lutz Beat B Bindila Laura L
Journal of lipid research 20171205 2
In this study, we report the development of a dual extraction protocol for RNA and lipids, including phospholipids, endocannabinoids, and arachidonic acid, at high spatial resolution, e.g., brain punches obtained from whole frozen brains corresponding to four brain subregions: dorsal hippocampus, ventral hippocampus, basolateral amygdala, and hypothalamus. This extraction method combined with LC/multiple reaction monitoring for lipid quantifi-cation and quantitative PCR for RNA investigation all ...[more]