Unknown

Dataset Information

0

Purification and biochemical characterization of an extracellular ?-d-fructofuranosidase from Aspergillus sp.


ABSTRACT: This study focused on the purification and characterization of an extracellular ?-d-fructofuranosidase or invertase from Aspergillus sojae JU12. The protein was purified by size exclusion chromatography with 5.41 fold and 10.87% recovery. The apparent molecular mass of the enzyme was estimated to be ~ 35 kDa using SDS-PAGE and confirmed by deconvoluted mass spectrometry. The fungal ?-d-fructofuranosidase was suggested to be a monomer by native PAGE and zymography, and was found to be a glycoprotein possessing 68.92% carbohydrate content. The products of enzyme hydrolysis were detected by thin layer chromatography and revealed the monosaccharide units, d-glucose and d-fructose. ?-d-fructofuranosidase showed enhanced activity at broad pH 4.0-9.0 and activity at a temperature range from 30 to 70 °C, while the enzyme was stable at pH 8.0 and 40 °C, respectively. The ?-d-fructofuranosidase activity was lowered by metal ion inhibitors Ag2+ and Hg2+ whereas elevated by SDS and ?-ME. The fungal ?-d-fructofuranosidase was capable of hydrolyzing d-sucrose and the kinetics were determined by Lineweaver-Burk plot with Km of 10.17 mM and Vmax of 0.7801 µmol min-1. Additionally, the extracellular ?-d-fructofuranosidase demonstrated tolerance to high ethanol concentrations indicating its applicability in the production of alcoholic fermentation processes.

SUBMITTER: Lincoln L 

PROVIDER: S-EPMC5794676 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Purification and biochemical characterization of an extracellular β-d-fructofuranosidase from <i>Aspergillus</i> sp.

Lincoln Lynette L   More Sunil S SS  

3 Biotech 20180120 2


This study focused on the purification and characterization of an extracellular β-d-fructofuranosidase or invertase from <i>Aspergillus sojae</i> JU12. The protein was purified by size exclusion chromatography with 5.41 fold and 10.87% recovery. The apparent molecular mass of the enzyme was estimated to be ~ 35 kDa using SDS-PAGE and confirmed by deconvoluted mass spectrometry. The fungal β-d-fructofuranosidase was suggested to be a monomer by native PAGE and zymography, and was found to be a gl  ...[more]

Similar Datasets

| S-EPMC7527383 | biostudies-literature
| S-EPMC7991022 | biostudies-literature
| S-EPMC2643559 | biostudies-literature
| S-EPMC6766936 | biostudies-literature
| S-EPMC7350912 | biostudies-literature
| S-EPMC7270473 | biostudies-literature
| S-EPMC5891470 | biostudies-literature
| S-EPMC4743132 | biostudies-literature
| S-EPMC3622683 | biostudies-literature
| S-EPMC5658581 | biostudies-literature