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ABSTRACT:
SUBMITTER: Andrianova M
PROVIDER: S-EPMC5795474 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Andrianova Mariia M Komarova Natalia N Grudtsov Vitaliy V Kuznetsov Evgeniy E Kuznetsov Alexander A
Sensors (Basel, Switzerland) 20171226 1
The electrochemical detection of interactions between aptamers and low-molecular-weight targets often lacks sensitivity. Signal amplification improves the detection of the aptamer-analyte complex; Bsm DNA polymerase was used to amplify the signal from the interaction of vanillin and its aptamer named Van_74 on an ion-sensitive field-effect transistor (ISFET)-based biosensor. The aptamer was immobilized on the ISFET sensitive surface. A short DNA probe was hybridized with the aptamer and dissocia ...[more]