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Neuroprotection of the hypoxic-ischemic mouse brain by human CD117+CD90+CD105+ amniotic fluid stem cells.


ABSTRACT: Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and this lack of knowledge limits their translational applications. Here, we show that SS-hAFSCs and RS-hAFSCs differ in their neuro-protective ability, demonstrating that a single contralateral injection of SS-hAFSCs into hypoxic-ischemic P7 mice conferred a 47% reduction in hippocampal tissue loss and 43-45% reduction in TUNEL-positive cells in the hippocampus and striatum 48 hours after the insult, decreased microglial activation and TGFβ1 levels, and prevented demyelination. On the other hand, RS-hAFSCs failed to show such neuro-protective effects. It is possible that SS-hAFSCs exert their neuroprotection via endoglin-dependent inhibition of TGFβ1 signaling in target cells. These findings identify a sub-population of CD117+CD90+CD105+ stem cells as a promising source for the neuro-protection of the developing brain.

SUBMITTER: Corcelli M 

PROVIDER: S-EPMC5799160 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Neuroprotection of the hypoxic-ischemic mouse brain by human CD117<sup>+</sup>CD90<sup>+</sup>CD105<sup>+</sup> amniotic fluid stem cells.

Corcelli Michelangelo M   Hawkins Kate K   Vlahova Filipa F   Hunjan Avina A   Dowding Kate K   De Coppi Paolo P   David Anna L AL   Peebles Donald D   Gressens Pierre P   Hagberg Henrik H   Hristova Mariya M   Guillot Pascale V PV  

Scientific reports 20180205 1


Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and this lack of knowledge limits their translational applications. Here, we show that SS-hAFSCs and RS-hAFSCs differ in their neuro-protective ability, demonstrating that a single contralateral injection o  ...[more]

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