Research Techniques Made Simple: Methodology and Applications of Forster Resonance Energy Transfer (FRET) Microscopy.

ABSTRACT: Classical biochemical techniques have contributed a great deal to our understanding of the mechanisms regulating fundamental biological processes. However, these approaches are typically end-point, population-based assays and are often insufficient in examining transient molecular events. Förster resonance energy transfer (FRET) microscopy is a powerful technique capable of investigating dynamic interactions between proteins and a plethora of biochemical signaling events based on the development of specific biosensors. This technique exploits the principle that when FRET occurs, energy from a donor fluorophore is transferred to an acceptor fluorophore only when certain conditions are met. These include dependence on both distance and fluorophore orientation. In this article, applications o

SUBMITTER: Broussard JA 

PROVIDER: S-EPMC5800528 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature