Dataset Information

Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq.

ABSTRACT: OBJECTIVE:Uighur medicine Fufang Munziq granule (Munziq) [corrected] shows cardioprotective effect in myocardial ischemia/reperfusion injury (IRI) in animal models, but the molecular mechanism of this effect is not clear. The present study investigates the regulation of nuclear factor kappa B (NF-kappa B) pathway by Fufang Munziq granule (Munziq) in IRI rat models. METHODS:Male Sprague-Dawley rats were divided into three groups: the NF-?B gene knockout group (n = 5); the NF-?B transgenic group (n = 8); and the control group (n = 5). All rats were treated with Fufang Munziq granule (Munziq) [corrected] for 21 days before underwent IRI surgical procedure. Blood and tissue samples were collected for the RT-PCR, ELISA, western blot and other examination. RESULTS:Expression of NF-?B p65mRNA and protein were down-regulated in the NF-?B knockout rats but up-regulated in the transgenic rats comparing with the controls (P<0.05). The upstream NF-?B kinase expressions, the downstream inflammatory cytokines, and the myocardial injury markers were all changed in accordance with the NF-?B gene modification (all P values <0.05). AMSq treatment relieved IRI in the NF-?B knockout rats. CONCLUSION:Inhibition on NF-?B signaling pathway may alleviate IRI in rats under Fufang Munziq granule (Munziq) [corrected] treatment.

SUBMITTER: Maimaitiaili A

PROVIDER: S-EPMC5801348 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq.

Maimaitiaili Abudunaibi A Li Junhong J Aibibula Aili A Abudureheman Mulati M

American journal of translational research 20180115 1

<h4>Objective</h4>Uighur medicine Fufang Munziq granule (Munziq) [corrected] shows cardioprotective effect in myocardial ischemia/reperfusion injury (IRI) in animal models, but the molecular mechanism of this effect is not clear. The present study investigates the regulation of nuclear factor kappa B (NF-kappa B) pathway by Fufang Munziq granule (Munziq) in IRI rat models.<h4>Methods</h4>Male Sprague-Dawley rats were divided into three groups: the NF-κB gene knockout group (n = 5); the NF-κB tra ...[more]

PMID: 29422995

Similar Datasets

Project description:Background:Oxidative stress is a major contributor to the onset and development of myocardial ischemia reperfusion injury (MIRI). Sappanone A (SA), a homoisoflavanone extracted from the heartwood of Caesalpinia sappan L., has been demonstrated to possess powerful antioxidant activity. Therefore, this study aimed to determine the protective effect of SA on MIRI and investigate its underlying mechanism. Methods:The rat hearts were isolated and underwent 30-min ischemia, followed by 120-min reperfusion to establish the MIRI model, using the Langendorff method. SA was administrated intraperitoneally into rats 1 h prior to heart isolation. The myocardial infarct size and apoptosis were measured by TTC and terminal deoxynucleotidyl transferase dUTP nick end labeling staining. Myocardial enzyme activity, MDA content and the activities of SOD and GSH-Px were detected by colorimetric spectrophotometric method. Reactive oxygen species (ROS) level was detected by DCFH-DA probe. The change in Keap1/Nrf2 signaling pathway was evaluated by Western blotting. Results:SA reduced myocardial infarct size and the release of CK-MB and LDH in a dose-dependent manner. Moreover, SA improved the recovery of cardiac function, inhibited MIRI-induced apoptosis, repressed the production of ROS and MDA, and enhanced the activities of SOD and GSH-Px. Mechanistically, SA downregulated Keap1, induced Nrf2 nuclear accumulation, and enhanced Nrf2 transcriptional activity, subsequently resulting in an increase in the expression of the Nrf2 target genes heme oxygenase-1 and NAD(P)H quinone dehydrogenase 1. Moreover, SA enhanced the phosphorylation of Nfr2, but the enhancement in Nfr2 phosphorylation was abrogated by PKC or PI3K inhibitor. Conclusion:Collectively, it was demonstrated that SA prevents MIRI via coordinating the cellular antioxidant defenses and maintaining the redox balance, by modulation of Nrf2 via the PKC or PI3K pathway. Therefore, SA was a potential therapeutic drug for treating MIRI.

Dataset Information

Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq.

Publications

Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets