Ontology highlight
ABSTRACT:
SUBMITTER: Chevalier A
PROVIDER: S-EPMC5802399 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Chevalier Aaron A Silva Daniel-Adriano DA Rocklin Gabriel J GJ Hicks Derrick R DR Vergara Renan R Murapa Patience P Bernard Steffen M SM Zhang Lu L Lam Kwok-Ho KH Yao Guorui G Bahl Christopher D CD Miyashita Shin-Ichiro SI Goreshnik Inna I Fuller James T JT Koday Merika T MT Jenkins Cody M CM Colvin Tom T Carter Lauren L Bohn Alan A Bryan Cassie M CM Fernández-Velasco D Alejandro DA Stewart Lance L Dong Min M Huang Xuhui X Jin Rongsheng R Wilson Ian A IA Fuller Deborah H DH Baker David D
Nature 20170927 7674
De novo protein design holds promise for creating small stable proteins with shapes customized to bind therapeutic targets. We describe a massively parallel approach for designing, manufacturing and screening mini-protein binders, integrating large-scale computational design, oligonucleotide synthesis, yeast display screening and next-generation sequencing. We designed and tested 22,660 mini-proteins of 37-43 residues that target influenza haemagglutinin and botulinum neurotoxin B, along with 6, ...[more]