Ontology highlight
ABSTRACT:
SUBMITTER: Nagendran M
PROVIDER: S-EPMC5802843 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Nagendran Monica M Riordan Daniel P DP Harbury Pehr B PB Desai Tushar J TJ
eLife 20180110
A major challenge in biology is identifying distinct cell classes and mapping their interactions in vivo. Tissue-dissociative technologies enable deep single cell molecular profiling but do not provide spatial information. We developed a proximity ligation in situ hybridization technology (PLISH) with exceptional signal strength, specificity, and sensitivity in tissue. Multiplexed data sets can be acquired using barcoded probes and rapid label-image-erase cycles, with automated calculation of si ...[more]