Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transplantation of schistosome sporocysts between host snails: A video guide.

ABSTRACT: Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies.

SUBMITTER: Mouahid G

PROVIDER: S-EPMC5806052 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Transplantation of <i>schistosome</i> sporocysts between host snails: A video guide.

Mouahid Gabriel G Rognon Anne A de Carvalho Augusto Ronaldo R Driguez Patrick P Geyer Kathy K Karinshak Shannon S Luviano Nelia N Mann Victoria V Quack Thomas T Rawlinson Kate K Wendt George G Grunau Christoph C Moné Hélène H

Wellcome open research 20180109

Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different <i>Schistosoma</i> species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and ...[more]

PMID: 29487916

Similar Datasets

FREP3 is a determinant of resistance to schistosome infection in snails

2011-07-30 | GSE28293 | GEO

FREP3 is a determinant of resistance to schistosome infection in snails (part 2)

Project description:Transcriptional profile of BS-90 snails injected with a cocktail of four FREP3 specific 27-mer DSiRNA oligos and two hours later exposed to S. mansoni miricidia. Compared to BS-90 snails injected with a cocktail of three GFP specific DSiRNA oligos and two hours later exposed to S. mansoni miricidia. Experiments were done over the course of 49 days. Snails were collected (10each) at 2 and 4 dpe to S. mansoni for comparison.

2012-04-01 | GSE33525 | GEO

FREP3 is a determinant of resistance to schistosome infection in snails

Project description:Transcriptional profile of snails exposed to irradiated E. paraensei miricidia and four days later challenged with S. mansoni miricidia. Compared to snails exposed to only irradiated E. paraensei miricidia. Each replicate is comprised of 1 individual snail from the specified treatment group. Six replicates of each treatment were analyzed on the Snail oligo array.

2011-07-29 | E-GEOD-28293 | biostudies-arrayexpress

Is host-schistosome coevolution going anywhere?

Project description:BACKGROUND:That pathogens and hosts coevolve is a powerful concept with broad theoretical and applied implications spanning from genetic theory to the medical and veterinary sciences, particularly in the context of infectious disease epidemiology. A substantial body of theory has been developed to explore the likelihood and consequences of coevolution, but few empirical studies have been conducted to test these theories, particularly for indirectly-transmitted pathogen-host systems. We initiated replicate longitudinal host-schistosome co-selection trials under different host genotype combinations: Schistosoma mansoni parasite lines were co-selected with populations of either previously resistant-selected Biomphalaria glabrata host genotypes, or unselected susceptible B. glabrata genotypes, or a mixed population of the two. All parasite lines were also passaged through their obligatory mammalian definitive host at each generation. RESULTS:We demonstrated variation in, and a reciprocal impact on, the fitness of both host and pathogen phenotype and genotype, an outcome dependent on the combinations of genotypes involved, and evidence of change over time. Most apparent was the observation that parasites appeared to rapidly adapt to those intermediate hosts previously selected for resistance. CONCLUSION:Our results illustrate the potential for host-schistosome coevolution and, in particular, suggest that host resistance may be a temporary phenomenon in nature due, in part, to rapid counter-adaptations by parasites.

| S-EPMC1920509 | biostudies-literature

FREP3 is a determinant of resistance to schistosome infection in snails (part 2)

2012-03-31 | E-GEOD-33525 | biostudies-arrayexpress

Effects of Endosulfan on Predator-Prey Interactions Between Catfish and Schistosoma Host Snails.

Project description:The effect of the pesticide endosulfan on predator-prey interactions between catfish and Schistosoma host snails was assessed in static tank experiments. Hybrid catfish (Clarias gariepinus × C. ngamensis) and Bulinus globosus were subjected to various endosulfan concentrations including an untreated control. The 48- and 96-h LC50 values for catfish were 1.0 and <0.5 µg/L, respectively, whereas the 48- and 96-h LC50 values for snails were 1137 and 810 µg/L. To assess sublethal effects on the feeding of the catfish on B. globosus, endosulfan concentrations between 0.03 and 1.0 µg/L were used. Predation was significantly greater (p < 0.001) in control tanks than in all other treatments. There was progressively decreasing predation with increasing toxicant concentration. Biological control of Schistosoma host snails using fish may be affected in endosulfan-polluted aquatic systems of Southern Africa because it has been found present at concentrations that are indicated to cause lethal effects on the evaluated hybrid catfish and to inhibit the predation of snails by this hybrid catfish.

| S-EPMC4935736 | biostudies-literature

Systematic Review and Meta-Analysis on the Infection Rates of Schistosome Transmitting Snails in Southern Africa.

Project description:Efforts to interrupt and eliminate schistosomiasis as a public health problem have increased in several Southern African countries. A systematic review was carried out on the infection rates of snails that cause schistosomiasis in humans. The searches were conducted in PubMed, Web of Science, and Scopus databases, using the PRISMA guidelines from inception to 24 February 2022. The study quality was assessed by using the Joanna Briggs Institute prevalence critical appraisal checklist. Pooled infection rates were estimated by using an inverse variance heterogeneity model, while heterogeneity was determined by using Cochran's Q test and Higgins i2 statistics. A total of 572 articles were screened, but only 28 studies were eligible for inclusion based on predetermined criteria. In the selected studies, 82,471 Bulinus spp. and 16,784 Biomphalaria spp. snails were screened for cercariae. The pooled infectivity of schistosome intermediate host snails, Biomphalaria spp., and Bulinus spp. were 1%, 2%, and 1%, respectively. Snail infection rates were higher in the 1900s compared to the 2000s. A Luis Furuya-Kanamori index of 3.16 indicated publication bias, and a high level of heterogeneity was observed. Although snail infectivity in Southern Africa is relatively low, it falls within the interval of common snail infection rates, thus indicating the need for suitable snail control programs that could interrupt transmission and achieve elimination.

| S-EPMC9145527 | biostudies-literature

A quick guide to video-tracking birds.

Project description:Video tracking is a powerful new tool for studying natural undisturbed behaviour in a wide range of birds, mammals and reptiles. Using integrated animal-borne video tags, video footage and positional data are recorded simultaneously from wild free-ranging animals. At the analysis stage, video scenes are linked to radio fixes, yielding an animal's eye view of resource use and social interactions along a known movement trajectory. Here, we provide a brief description of our basic equipment and field techniques to enable other researchers to start their own video-tracking studies.

| S-EPMC2610131 | biostudies-literature

Differential expression of microRNAs in the non-permissive schistosome host Microtus fortis under schistosome infection.

Project description:The reed vole Microtus fortis is the only mammal known in China in which the growth, development and maturation of schistosomes (Schistosoma japonicum) is prevented. It might be that the anti-schistosomiasis mechanisms of M. fortis associate with microRNA-mediated gene expression, given that the latter has been found to be involved in gene regulation in eukaryotes. In the present study, the difference between pathological changes in tissues of M. fortis and of mice (Mus musculus) post-schistosome infection were observed by using hematoxylin-eosin staining. In addition, microarray technique was applied to identify differentially expressed miRNAs in the same tissues before and post-infection to analyze the potential roles of miRNAs in schistosome infection in these two different types of host. Histological analyses showed that S. japonicum infection in M. fortis resulted in a more intensive inflammatory response and pathological change than in mice. The microarray analysis revealed that 162 miRNAs were expressed in both species, with 12 in liver, 32 in spleen and 34 in lung being differentially expressed in M. fortis. The functions of the differentially expressed miRNAs were mainly revolved in nutrient metabolism, immune regulation, etc. Further analysis revealed that important signaling pathways were triggered after infection by S. japonicum in M. fortis but not in the mice. These results provide new insights into the general mechanisms of regulation in the non-permissive schistosome host M. fortis that exploits potential miRNA regulatory networks. Such information will help improve current understanding of schistosome development and host-parasite interactions.

| S-EPMC3877346 | biostudies-literature

Stem cells rejuvinate the schistosome host-parasite interface

Project description:We report the transcriptional effects of long-term stem cell depletion in Schistosoma mansoni

2015-12-10 | GSE75861 | GEO