Ontology highlight
ABSTRACT:
SUBMITTER: Sanjo H
PROVIDER: S-EPMC5809087 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Sanjo Hiroyuki H Komeya Mitsuru M Sato Takuya T Abe Takeru T Katagiri Kumiko K Yamanaka Hiroyuki H Ino Yoko Y Arakawa Noriaki N Hirano Hisashi H Yao Tatsuma T Asayama Yuta Y Matsuhisa Akio A Yao Masahiro M Ogawa Takehiko T
PloS one 20180212 2
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential medium (α-MEM), supplemented with Knockout serum replacement (KSR) or AlbuMAX, neither of which were defined chemically. In this study, we formulated a chemically defined medium (CDM) that can induce mouse spermatogenesis under organ culture conditions. It was found that bovine serum albumin (BSA) purified through three ...[more]