Ontology highlight
ABSTRACT:
SUBMITTER: Bulanova DR
PROVIDER: S-EPMC5810789 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Bulanova Daria R DR Akimov Yevhen A YA Rokka Anne A Laajala Teemu D TD Aittokallio Tero T Kouvonen Petri P Pellinen Teijo T Kuznetsov Sergey G SG
Cell adhesion & migration 20170206 5-6
G-Protein Coupled Receptor (GPCR), Class C, Group 5, Member A (GPRC5A) has been implicated in several malignancies. The underlying mechanisms, however, remain poorly understood. Using a panel of human cell lines, we demonstrate that CRISPR/Cas9-mediated knockout and RNAi-mediated depletion of GPRC5A impairs cell adhesion to integrin substrates: collagens I and IV, fibronectin, as well as to extracellular matrix proteins derived from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma (Matrigel). Consi ...[more]