Ontology highlight
ABSTRACT:
SUBMITTER: Graybill RM
PROVIDER: S-EPMC5812356 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Graybill Richard M RM Para Christopher S CS Bailey Ryan C RC
Analytical chemistry 20161019 21
We describe an approach for multiplexed microRNA analysis using silicon photonic microring resonators to detect cDNA reverse transcription products via a subsequent enzymatic signal enhancement strategy. Key to this method is a modified stem loop primer that facilitates downstream signal amplification via enzymatic turnover and improves the sensor signal 20-fold when compared to traditional stem loop primers. This approach facilitates targeted microRNA quantification in only 2.5 h and without re ...[more]