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Ten Basic Rules of Antibody Validation.

ABSTRACT: The quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able to assess the validation level of an immunochemical reagent. A simple, 1-page checklist is supplied for the practical application of these criteria.

SUBMITTER: Weller MG

PROVIDER: S-EPMC5813849 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Ten Basic Rules of Antibody Validation.

Weller Michael G MG

Analytical chemistry insights 20180208

The quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able to assess the validation level of an immunochemical reagent. A simple, 1-page checklist is supplied for the practical application of these criteria. ...[more]

PMID: 29467569

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