Ontology highlight
ABSTRACT:
SUBMITTER: Vasta JD
PROVIDER: S-EPMC5814754 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Cell chemical biology 20171122 2
For kinase inhibitors, intracellular target selectivity is fundamental to pharmacological mechanism. Although a number of acellular techniques have been developed to measure kinase binding or enzymatic inhibition, such approaches can fail to accurately predict engagement in cells. Here we report the application of an energy transfer technique that enabled the first broad-spectrum, equilibrium-based approach to quantitatively profile target occupancy and compound affinity in live cells. Using thi ...[more]