Dataset Information

Effect of nanozeolite and plant growth promoting rhizobacteria on maize.

ABSTRACT: Plant growth promoting rhizobacteria are key to soil and plant health maintenance. In the present study, two PGPR strains which were identified as Bacillus spp. (accession number KX650178 and KX650179) with nanozeolite (50 ppm) were applied to the seeds in different combinations and tested on growth profile of maize crop. Various growth related parameters, including plant height, leaf area, number of leaves chlorophyll and total protein were positively increased up to twofold by the nanocompound treatment. GC-MS results reveal increase in total phenolic and acid ester compounds after the treatment of nanozeolite and PGPR, which are responsible for stress tolerance mechanism. Soil physicochemical parameters (organic carbon, phosphorous, potassium, ammoniacal nitrogen and nitrate nitrogen) were assessed qualitatively and a shift towards higher amount was observed. Various biochemical parameters of soil health like dehydrogenase, fluorescein diacetate hydrolysis and alkaline phosphatase activity were significantly enhanced up to threefold with the application of different treatments. The results, for the first time, demonstrate successful use of nanozeolite in enhancing growth of Zea mays, under controlled conditions and present a viable alternative to GM crop for ensuring food security.

SUBMITTER: Khati P

PROVIDER: S-EPMC5818361 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Effect of nanozeolite and plant growth promoting rhizobacteria on maize.

Khati Priyanka P Parul Bhatt Pankaj P Nisha Kumar Rajeew R Sharma Anita A

3 Biotech 20180219 3

Plant growth promoting rhizobacteria are key to soil and plant health maintenance. In the present study, two PGPR strains which were identified as Bacillus spp. (accession number KX650178 and KX650179) with nanozeolite (50 ppm) were applied to the seeds in different combinations and tested on growth profile of maize crop. Various growth related parameters, including plant height, leaf area, number of leaves chlorophyll and total protein were positively increased up to twofold by the nanoc ...[more]

PMID: 29484280

Similar Datasets

Project description:Plant growth-promoting rhizobacteria (PGPR) may provide a biological alternative to fix atmospheric N2 and delay N remobilisation in maize plant to increase crop yield, based on an understanding that plant-N remobilisation is directly correlated to its plant senescence. Thus, four PGPR strains were selected from a series of bacterial strains isolated from maize roots at two locations in Malaysia. The PGPR strains were screened in vitro for their biochemical plant growth-promoting (PGP) abilities and plant growth promotion assays. These strains were identified as Klebsiella sp. Br1, Klebsiella pneumoniae Fr1, Bacillus pumilus S1r1 and Acinetobacter sp. S3r2 and a reference strain used was Bacillus subtilis UPMB10. All the PGPR strains were tested positive for N2 fixation, phosphate solubilisation and auxin production by in vitro tests. In a greenhouse experiment with reduced fertiliser-N input (a third of recommended fertiliser-N rate), the N2 fixation abilities of PGPR in association with maize were determined by 15N isotope dilution technique at two harvests, namely, prior to anthesis (D50) and ear harvest (D65). The results indicated that dry biomass of top, root and ear, total N content and bacterial colonisations in non-rhizosphere, rhizosphere and endosphere of maize roots were influenced by PGPR inoculation. In particular, the plants inoculated with B. pumilus S1r1 generally outperformed those with the other treatments. They produced the highest N2 fixing capacity of 30.5% (262 mg N2 fixed plant-1) and 25.5% (304 mg N2 fixed plant-1) of the total N requirement of maize top at D50 and D65, respectively. N remobilisation and plant senescence in maize were delayed by PGPR inoculation, which is an indicative of greater grain production. This is indicated by significant interactions between PGPR strains and time of harvests for parameters on N uptake and at. % 15Ne of tassel. The phenomenon is also supported by the lower N content in tassels of maize treated with PGPR, namely, B. pumilus S1r1, K. pneumoniae Fr1, B. subtilis UPMB10 and Acinetobacter sp. S3r2 at D65 harvest. This study provides evidence that PGPR inoculation, namely, B. pumilus S1r1 can biologically fix atmospheric N2 and provide an alternative technique, besides plant breeding, to delay N remobilisation in maize plant for higher ear yield (up to 30.9%) with reduced fertiliser-N input.

Project description:Arabidopsis thaliana transcriptome analysis in response to plant growth promoting rhizobacteria (PGPR) Experiment 1 : Changes in gene expression profile triggered during root architecture response to Phyllobacterium. Biological question : Which genes are up- or down-regulated in Arabidopsis thaliana cultivated in vitro with increased lateral root development in response to Phyllobacterium STM196 inoculation. Experiment description: Seeds of wild-type Arabidopsis thaliana (ecotype Columbia) were surface-sterilized and sown on agar mineral medium (see below). 4 days after storage in the dark at 4C, seedling were cultivated 6 days in a growth chamber (16 h daily, 20-22C) and then transferred on a fresh agar mineral medium inoculated or not with Phyllobacterium STM196 (2.108 cfu/ml). 6 days later, root and leaves were collected, froze on liquid nitrogen and stored at -80C. Experiment 2 : Changes in gene expression profile triggered during induced systemic resistance (ISR) Biological question : Which genes are up- or down-regulated during the ISR triggered by a rhizobacteria, in comparison with those affected by a pathogenic interaction. Experiment description: Seeds were sown on 0.8% (W/V) agar mineral medium (see below). 4 days after storage in the dark at 4C, seedling were cultivated 6 days in a growth chamber (16 h daily, 20-22C) and then transferred on soil inoculated or not with 107 cfu.g-1 of Bradyrhizobium strain ORS278. Three weeks later, 3 leaves per plant were infiltrated with a suspension of Pseudomonas syringae pv. tomato (2.105 cfu.ml-1) or with MgSO4 10 mM alone for control plants. Infiltrated leaves were collected 24h later. Experiment 3 : Comparison of the effects of 3 rhizobacteria on Arabidopsis thaliana transcriptome Biological question : which genes are specifically induced or repressed in Arabidopsis thaliana by inoculation of the soil with a PGPR vs a bacteria that has the ability to trigger nodule formation in a Legume. Experiment description: Seeds of wild-type Arabidopsis thaliana (ecotype Columbia) were surface-sterilized and sown on agar mineral medium. Four days after storage in the dark at 4C, seedlings were cultivated 6 days in a growth chamber (16 h daily, 20-22C) and then transferred on soil inoculated or not with 108 cfu.g-1 of Mesorhizobium loti, or 108 cfu.g-1 of Phyllobacterium STM196, or 107 cfu.g-1 of Bradyrhizobium ORS278.

Dataset Information

Effect of nanozeolite and plant growth promoting rhizobacteria on maize.

Publications

Effect of nanozeolite and plant growth promoting rhizobacteria on maize.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets