Profiling the transcriptome with RNA SPOTs.
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ABSTRACT:
SUBMITTER: Eng CL
PROVIDER: S-EPMC5819366 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Publications
Profiling the transcriptome with RNA SPOTs.
Eng Chee-Huat Linus CL Shah Sheel S Thomassie Julian J Cai Long L
Nature methods 20171113 12
Single-molecule FISH (smFISH) has been the gold standard for quantifying individual transcript abundances. Here, we scale up multiplexed smFISH to the transcriptome level and profile 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This method, called RNA sequential probing of targets (SPOTs), provides an accurate, flexible, and low-cost alternative to sequencing for profiling transcriptomes. ...[more]
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