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Systematic Identification of Intracellular-Translocated Candidate Effectors in Edwardsiella piscicida.


ABSTRACT: Many bacterial pathogens inject effectors directly into host cells to target a variety of host cellular processes and promote bacterial dissemination and survival. Identifying the bacterial effectors and elucidating their functions are central to understanding the molecular pathogenesis of these pathogens. Edwardsiella piscicida is a pathogen with a wide host range, and very few of its effectors have been identified to date. Here, based on the genes significantly regulated by macrophage infection, we identified 25 intracellular translocation-positive candidate effectors, including all five previously reported effectors, namely EseG, EseJ, EseH, EseK, and EvpP. A subsequent secretion analysis revealed diverse secretion patterns for the 25 effector candidates, suggesting that multiple transport pathways were involved in the internalization of these candidate effectors. Further, we identified two novel type VI secretion system (T6SS) putative effectors and three outer membrane vesicles (OMV)-dependent putative effectors among the candidate effectors described above, and further analyzed their contribution to bacterial virulence in a zebrafish model. This work demonstrates an effective approach for screening bacterial effectors and expands the effectors repertoire in E. piscicida.

SUBMITTER: Zhang L 

PROVIDER: S-EPMC5820615 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Systematic Identification of Intracellular-Translocated Candidate Effectors in <i>Edwardsiella piscicida</i>.

Zhang Lingzhi L   Jiang Zhiwei Z   Fang Shan S   Huang Yajun Y   Yang Dahai D   Wang Qiyao Q   Zhang Yuanxing Y   Liu Qin Q  

Frontiers in cellular and infection microbiology 20180216


Many bacterial pathogens inject effectors directly into host cells to target a variety of host cellular processes and promote bacterial dissemination and survival. Identifying the bacterial effectors and elucidating their functions are central to understanding the molecular pathogenesis of these pathogens. <i>Edwardsiella piscicida</i> is a pathogen with a wide host range, and very few of its effectors have been identified to date. Here, based on the genes significantly regulated by macrophage i  ...[more]

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