Dataset Information

First Report of bla_CTX-M-28 in Enterobacteriaceae Isolates in the United Arab Emirates.

ABSTRACT:

Background

The CTX-M family of extended-spectrum beta lactamase (ESBL) enzymes is comprised of over 60 bla_CTX-M gene variants with the predominance of bla_CTX-M-15 in many regions. In this report, we present the first description of bla_CTX-M-28 in the United Arab Emirates.

Methods

Forty-five non-duplicate ESBL producing isolates identified in a secondary care facility in the United Arab Emirates from June to July 2016 were studied. Gene sequencing was performed and DNA sequences were annotated using the BLAST program to identify the gene subtypes.

Results

The majority of the ESBL positive isolates were E. coli (n/N = 39/45; 86.6%) followed by K. pneumoniae (n = 5) and K. oxytoca (n = 1). All isolates harboured bla_CTX-M and bla_TEM genes, 18 had bla_SHV, and 2 were bla_VIM positive. Thirty-seven isolates (82.2%) were positive for bla_CTX-M-28. Other bla_CTX-M genes identified include bla_CTX-M-167 (n = 2; isolates #1 and 26) and one each for bla_CTX-M-38, bla_CTX-M-163, and bla_CTX-M-198. No bla_CTX-M-15 was identified. The predominant bla_TEM subtype was bla_TEM-171 (n = 8) followed by one of each of bla_TEM-120, bla_TEM-163, and bla_TEM-206. The bla_SHV subtypes were bla_SHV-148 and bla_SHV-187.

Conclusion

The findings indicate the first description of bla_CTX-M-28 in a setting where bla_CTX-M-15 was previously predominant.

SUBMITTER: Alfaresi M

PROVIDER: S-EPMC5822763 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

First Report of <i>bla</i><sub>CTX-M-28</sub> in Enterobacteriaceae Isolates in the United Arab Emirates.

Alfaresi Mubarak M Kim Sing Garwin G Senok Abiola A

Journal of pathogens 20180118

<h4>Background</h4>The CTX-M family of extended-spectrum beta lactamase (ESBL) enzymes is comprised of over 60 <i>bla</i><sub>CTX-M</sub> gene variants with the predominance of <i>bla</i><sub>CTX-M-15</sub> in many regions. In this report, we present the first description of <i>bla</i><sub>CTX-M-28</sub> in the United Arab Emirates.<h4>Methods</h4>Forty-five non-duplicate ESBL producing isolates identified in a secondary care facility in the United Arab Emirates from June to July 2016 were studi ...[more]

PMID: 29593911

Similar Datasets

Project description:Enterobacteria that produce extended-spectrum β-lactamase (ESBL) such as Escherichia coli (E. coli) are common in our environment and known to cause serious health implications in humans and animals. β-lactam antibiotics such as penicillins, cephalosporins and monobactams are the most commonly used anti-bacterials in both humans and animals, however, Gram negative bacteria (such as E. coli) that produces extended-spectrum β-lactamases (ESBLs) have the ability to hydrolyze most β-lactams therefore making them resistant to β-lactam antibiotics. Recent extensive researches on the epidemiology and genetic characteristics of extended-spectrum β-lactamase (ESBL)-producing E. coli reported the existence of ESBL-producing E. coli in humans, companion animals and poultry. Therefore, this experiment was performed to investigate the prevalence and genetic characteristics of β-lactamase producing E. coli isolated from beef cattle farms in the Sichuan-Chongqing circle of China. Phenotypic confirmation of ESBL-producing E. coli was performed using the double disk synergy test. Polymerase Chain Reaction (PCR) was used to detect blaCTX-M, blaSHV and blaTEM gene codes, then after, isolates were divided into different phylogenetic groups and multi-locus sequence typing (MLST). The results showed that out of the 222 E. coli strains isolated from the beef cattle, 102 strains showed ESBL phenotypes. The PCR results showed that blaCTX-M was the predominant ESBL gene identified among the E. coli strains with 21 (9.5%) isolates having this gene, followed by blaSHV which was found in 18 (8.1%) isolates. The majority of these ESBL positive isolates were assigned to phylogroup A (19.8%) followed by phylogroup B1 (13.5%). In addition, from the MLST results on ESBL positive isolates (n = 30) we identified 19 STs, ST398 (ST398cplx) and ST7130 which were the prevalent population (20%). In conclusion, the high prevalence of CTX-M, and SHV in the study confirmed its association with E. coli infection; therefore, this calls for health concerns on ESBL-producing E. coli. As far as we know, this is the first comprehensive research report relating to ESBL-producing E. coli incidence in Chinese beef cattle.

Project description:BACKGROUND:Comprehensive global data on the health effects of indoor air pollutants are lacking. There are few large population-based multi-air pollutant health assessments. Further, little is known about indoor air health risks in the Middle East, especially in countries undergoing rapid economic development. OBJECTIVES:To provide multifactorial indoor air exposure and health data, we conducted a population-based study of indoor air pollution and health in the United Arab Emirates (UAE). METHODS:We conducted a cross-sectional study in a population-based sample of 628 households in the UAE. Indoor air pollutants [sulfur dioxide (SO2), nitrogen dioxide (NO2), hydrogen sulfide (H2S), formaldehyde (HCHO), carbon monoxide (CO), and particulate matter] were measured using passive samplers over a 7-day period. Health information was collected from 1,590 household members via in-person interviews. RESULTS:Participants in households with quantified SO2, NO2, and H2S (i.e., with measured concentrations above the limit of quantification) were twice as likely to report doctor-diagnosed asthma. Participants in homes with quantified SO2 were more likely to report wheezing symptoms {ever wheezing, prevalence odds ratio [POR] 1.79 [95% confidence interval (CI) 1.05, 3.05]; speech-limiting wheeze, POR 3.53 (95% CI: 1.06, 11.74)}. NO2 and H2S were similarly associated with wheezing symptoms. Quantified HCHO was associated with neurologic symptoms (difficulty concentrating POR 1.47; 95% CI: 1.02, 2.13). Burning incense daily was associated with increased headaches (POR 1.87; 95% CI: 1.09, 3.21), difficulty concentrating (POR 3.08; 95% CI: 1.70, 5.58), and forgetfulness (POR 2.68: 95% CI: 1.47, 4.89). CONCLUSIONS:This study provides new information regarding potential health risks from pollutants commonly found in indoor environments in the UAE and other countries. Multipollutant exposure and health assessments in cohort studies are needed to better characterize health effects of indoor air pollutants.