Bisdemethoxycurcumin and Its Cyclized Pyrazole Analogue Differentially Disrupt Lipopolysaccharide Signalling in Human Monocyte-Derived Macrophages.

ABSTRACT: Several studies suggest that curcumin and related compounds possess antioxidant and anti-inflammatory properties including modulation of lipopolysaccharide- (LPS-) mediated signalling in macrophage cell models. We here investigated the effects of curcumin and the two structurally unrelated analogues GG6 and GG9 in primary human blood-derived macrophages as well as the signalling pathways involved. Macrophages differentiated from peripheral blood monocytes for 7 days were activated with LPS or selective Toll-like receptor agonists for 24 h. The effects of test compounds on cytokine production and immunophenotypes evaluated as CD80⁺/CCR2⁺ and CD206⁺/CD163⁺ subsets were examined by ELISA and flow cytometry. Signalling pathways were probed by Western

SUBMITTER: Tedesco S 

PROVIDER: S-EPMC5822910 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature