Dataset Information

LRP1 expression in colon cancer predicts clinical outcome.

ABSTRACT: LRP1 (low-density lipoprotein receptor-related protein 1), a multifunctional endocytic receptor, has recently been identified as a hub within a biomarker network for multi-cancer clinical outcome prediction. As its role in colon cancer has not yet been characterized, we here investigate the relationship between LRP1 and outcome.

Materials and methods

LRP1 mRNA expression was determined in colon adenocarcinoma and paired colon mucosa samples, as well as in stromal and tumor cells obtained after laser capture microdissection. Clinical potential was further investigated by immunohistochemistry in a population-based colon cancer series (n = 307). LRP1 methylation, mutation and miR-205 expression were evaluated and compared with LRP1 expression levels.

Results

LRP1 mRNA levels were significantly lower in colon adenocarcinoma cells compared with colon mucosa and stromal cells obtained after laser capture microdissection. Low LRP1 immunohistochemical expression in adenocarcinomas was associated with higher age, right-sided tumor, loss of CDX2 expression, Annexin A10 expression, CIMP-H, MSI-H and BRAFV600E mutation. Low LRP1 expression correlated with poor clinical outcome, especially in stage IV patients. While LRP1 expression was downregulated by LRP1 mutation, LRP1 promoter was never methylated.

Conclusions

Loss of LRP1 expression is associated with worse colon cancer outcomes. Mechanistically, LRP1 mutation modulates LRP1 expression.

SUBMITTER: Boulagnon-Rombi C

PROVIDER: S-EPMC5823651 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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LRP1 expression in colon cancer predicts clinical outcome.

Boulagnon-Rombi Camille C Schneider Christophe C Leandri Chloé C Jeanne Albin A Grybek Virginie V Bressenot Aude Marchal AM Barbe Coralie C Marquet Benjamin B Nasri Saviz S Coquelet Christelle C Fichel Caroline C Bouland Nicole N Bonnomet Arnaud A Kianmanesh Reza R Lebre Anne-Sophie AS Bouché Olivier O Diebold Marie-Danièle MD Bellon Georges G Dedieu Stéphane S

Oncotarget 20180113 10

LRP1 (low-density lipoprotein receptor-related protein 1), a multifunctional endocytic receptor, has recently been identified as a hub within a biomarker network for multi-cancer clinical outcome prediction. As its role in colon cancer has not yet been characterized, we here investigate the relationship between LRP1 and outcome.<h4>Materials and methods</h4><i>LRP1</i> mRNA expression was determined in colon adenocarcinoma and paired colon mucosa samples, as well as in stromal and tumor cells ob ...[more]

PMID: 29507659

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Dataset Information

LRP1 expression in colon cancer predicts clinical outcome.

Materials and methods

Results

Conclusions

Publications

LRP1 expression in colon cancer predicts clinical outcome.

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