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G?i-mediated TRPC4 activation by polycystin-1 contributes to endothelial function via STAT1 activation.

ABSTRACT: Hypertension and aneurysm are frequently associated with autosomal dominant polycystic kidney disease (ADPKD) caused by polycystin-1 (PC1) mutations, which is closely related to endothelial dysfunction. PC1 is an atypical G-protein-coupled receptor that activates G-proteins by self-cleavage; currently, however, the molecular and cellular mechanisms of the associated intracellular signaling and ion channel activation remain poorly elucidated. Here, we report an activation mechanism of a calcium-permeable canonical transient receptor potential 4 (TRPC4) channel by PC1 and its endothelial function. We found that the inhibitory G?i3 protein selectively bound to the G-protein-binding domain on the C-terminus of PC1. The dissociation of G?i3 upon cleavage of PC1 increased TRPC4 activity. Calcium influx through TRPC4 activated the transcription factor STAT1 to regulate cell proliferation and death. The down-regulation of PC1/TRPC4/STAT1 disrupted migration of endothelial cell monolayers, leading to an increase in endothelial permeability. These findings contribute to greater understanding of the high risk of aneurysm in patients with ADPKD.

SUBMITTER: Kwak M

PROVIDER: S-EPMC5823873 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Gα<sub>i</sub>-mediated TRPC4 activation by polycystin-1 contributes to endothelial function via STAT1 activation.

Kwak Misun M Hong Chansik C Myeong Jongyun J Park Eunice Yon June EYJ Jeon Ju-Hong JH So Insuk I

Scientific reports 20180222 1

Hypertension and aneurysm are frequently associated with autosomal dominant polycystic kidney disease (ADPKD) caused by polycystin-1 (PC1) mutations, which is closely related to endothelial dysfunction. PC1 is an atypical G-protein-coupled receptor that activates G-proteins by self-cleavage; currently, however, the molecular and cellular mechanisms of the associated intracellular signaling and ion channel activation remain poorly elucidated. Here, we report an activation mechanism of a calcium-p ...[more]

PMID: 29472562

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Dataset Information

G?i-mediated TRPC4 activation by polycystin-1 contributes to endothelial function via STAT1 activation.

Publications

Gα<sub>i</sub>-mediated TRPC4 activation by polycystin-1 contributes to endothelial function via STAT1 activation.

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