Dataset Information

Metabolite profiling with HPLC-ICP-MS as a tool for in vivo characterization of imaging probes.

ABSTRACT:

Background

Current analytical methods for characterizing pharmacokinetic and metabolic properties of positron emission tomography (PET) and single photon emission computed tomography (SPECT) probes are limited. Alternative methods to study tracer metabolism are needed. The study objective was to assess the potential of high performance liquid chromatography - inductively coupled plasma - mass spectrometry (HPLC-ICP-MS) for quantification of molecular probe metabolism and pharmacokinetics using stable isotopes.

Methods

Two known peptide-DOTA conjugates were chelated with ^natGa and ^natIn. Limit of detection of HPLC-ICP-MS for ⁶⁹Ga and ¹¹⁵In was determined. Rats were administered 50-150 nmol of Ga- and/or In-labeled probes, blood was serially sampled, and plasma analyzed by HPLC-ICP-MS using both reverse phase and size exclusion chromatography.

Results

The limits of detection were 0.16 pmol for ¹¹⁵In and 0.53 pmol for ⁶⁹Ga. Metabolites as low as 0.001 %ID/g could be detected and transchelation products identified. Simultaneous administration of Ga- and In-labeled probes allowed the determination of pharmacokinetics and metabolism of both probes in a single animal.

Conclusions

HPLC-ICP-MS is a robust, sensitive and radiation-free technique to characterize the pharmacokinetics and metabolism of imaging probes.

SUBMITTER: Boros E

PROVIDER: S-EPMC5824709 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Metabolite profiling with HPLC-ICP-MS as a tool for in vivo characterization of imaging probes.

Boros Eszter E Pinkhasov Omar R OR Caravan Peter P

EJNMMI radiopharmacy and chemistry 20180122 1

<h4>Background</h4>Current analytical methods for characterizing pharmacokinetic and metabolic properties of positron emission tomography (PET) and single photon emission computed tomography (SPECT) probes are limited. Alternative methods to study tracer metabolism are needed. The study objective was to assess the potential of high performance liquid chromatography - inductively coupled plasma - mass spectrometry (HPLC-ICP-MS) for quantification of molecular probe metabolism and pharmacokinetics ...[more]

PMID: 29503859

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Dataset Information

Metabolite profiling with HPLC-ICP-MS as a tool for in vivo characterization of imaging probes.

Background

Methods

Results

Conclusions

Publications

Metabolite profiling with HPLC-ICP-MS as a tool for in vivo characterization of imaging probes.

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