Ontology highlight
ABSTRACT:
SUBMITTER: Cheng WWL
PROVIDER: S-EPMC5827446 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Cheng Wayland W L WWL Chen Zi-Wei ZW Bracamontes John R JR Budelier Melissa M MM Krishnan Kathiresan K Shin Daniel J DJ Wang Cunde C Jiang Xin X Covey Douglas F DF Akk Gustav G Evers Alex S AS
The Journal of biological chemistry 20180104 8
Neurosteroids are endogenous sterols that potentiate or inhibit pentameric ligand-gated ion channels (pLGICs) and can be effective anesthetics, analgesics, or anti-epileptic drugs. The complex effects of neurosteroids on pLGICs suggest the presence of multiple binding sites in these receptors. Here, using a series of novel neurosteroid-photolabeling reagents combined with top-down and middle-down mass spectrometry, we have determined the stoichiometry, sites, and orientation of binding for 3α,5α ...[more]