Ontology highlight
ABSTRACT:
SUBMITTER: Shen H
PROVIDER: S-EPMC5827971 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Shen Hongying H Campanello Gregory C GC Flicker Daniel D Grabarek Zenon Z Hu Junchi J Luo Cheng C Banerjee Ruma R Mootha Vamsi K VK
Cell 20171019 4
CLYBL encodes a ubiquitously expressed mitochondrial enzyme, conserved across all vertebrates, whose cellular activity and pathway assignment are unknown. Its homozygous loss is tolerated in seemingly healthy individuals, with reduced circulating B<sub>12</sub> levels being the only and consistent phenotype reported to date. Here, by combining enzymology, structural biology, and activity-based metabolomics, we report that CLYBL operates as a citramalyl-CoA lyase in mammalian cells. Cells lacking ...[more]