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Update of thermotolerant genes essential for survival at a critical high temperature in Escherichia coli.

ABSTRACT: Previous screening of a single-gene knockout library consisting of 3,908 disrupted-mutant strains allowed us to identify 51 thermotolerant genes that are essential for survival at a critical high temperature (CHT) in Escherichia coli [Murata M, Fujimoto H, Nishimura K, Charoensuk K, Nagamitsu H, Raina S, Kosaka T, Oshima T, Ogasawara N, Yamada M (2011) PLoS ONE 6: e20063]. In this study, we identified another 21 thermotolerant genes. E. coli thus has 72 thermotolerant genes in total. The genes are classified into 8 groups: genes for energy metabolism, outer membrane organization, DNA double-strand break repair, tRNA modification, protein quality control, translation control, cell division and transporters. This classification and physiological analysis indicate the existence of fundamental strategies for survival at a CHT, which seems to exclude most of the heat shock responses.

SUBMITTER: Murata M

PROVIDER: S-EPMC5828445 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Update of thermotolerant genes essential for survival at a critical high temperature in Escherichia coli.

Murata Masayuki M Ishii Ayana A Fujimoto Hiroko H Nishimura Kaori K Kosaka Tomoyuki T Mori Hirotada H Yamada Mamoru M

PloS one 20180227 2

Previous screening of a single-gene knockout library consisting of 3,908 disrupted-mutant strains allowed us to identify 51 thermotolerant genes that are essential for survival at a critical high temperature (CHT) in Escherichia coli [Murata M, Fujimoto H, Nishimura K, Charoensuk K, Nagamitsu H, Raina S, Kosaka T, Oshima T, Ogasawara N, Yamada M (2011) PLoS ONE 6: e20063]. In this study, we identified another 21 thermotolerant genes. E. coli thus has 72 thermotolerant genes in total. The genes a ...[more]

PMID: 29485997

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