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Indigenous plant growth-promoting bacteria enhance plant growth, biomass, and nutrient uptake in degraded forest plants.

ABSTRACT: The present study was aimed to isolate indigenous plant growth-promoting bacteria (PGPB) from Nanmangalam reserve forest, India and to analyze their positive impact on nursery plant species. In total, 160 isolates were obtained from different nitrogen-free Media (LGI, JMV, NFB). Amongst these, 12 isolates were shown positive for 5-8% of ammonia production nif H positive and then isolates were further tested for their plant growth-promoting (PGP) activity. Based on their PGP activity, nine isolates were selected, and applied in nurseries of twelve native plant species, along with organic manure and inorganic fertilizer. All the isolates were shown positive effects when compared to control. In that, five of these bacterial isolates, Paenibacillus sp. RRB2, Azospirillum brasilense RRAK5, Bacillus subtilis subsp. subtilis RRD8, Burholdria kururiensis RRAK1, and Pseudomonas stutzeri RRAN2, enhanced biomass production in several trees.

SUBMITTER: Radhapriya P

PROVIDER: S-EPMC5829267 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Indigenous plant growth-promoting bacteria enhance plant growth, biomass, and nutrient uptake in degraded forest plants.

Radhapriya Parthasarathy P Ramachandran Andimuthu A Palani Perumal P

3 Biotech 20180228 3

The present study was aimed to isolate indigenous plant growth-promoting bacteria (PGPB) from Nanmangalam reserve forest, India and to analyze their positive impact on nursery plant species. In total, 160 isolates were obtained from different nitrogen-free Media (LGI, JMV, NFB). Amongst these, 12 isolates were shown positive for 5-8% of ammonia production nif H positive and then isolates were further tested for their plant growth-promoting (PGP) activity. Based on their PGP activity, nine isolat ...[more]

PMID: 29492373

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Project description:The study was aimed at assessing the effects of indigenous Plant Growth Promoting Bacterium (PGPB) on the legume Pongamia pinnata in the degraded soil of the Nanmangalam Reserve Forest (NRF) under nursery conditions. In total, 160 diazotrophs were isolated from three different nitrogen-free semi-solid media (LGI, Nfb, and JMV). Amongst these isolates, Pseudomonas aeruginosa RRALC3 exhibited the maximum ammonia production and hence was selected for further studies. RRALC3 was found to possess multiple plant growth promoting traits such as nitrogen accumulation (120.6ppm); it yielded a positive amplicon with nifH specific primers, tested positive for Indole Acetic Acid (IAA; 18.3μg/ml) and siderophore production, tested negative for HCN production and was observed to promote solubilization of phosphate, silicate and zinc in the plate assay. The 16S rDNA sequence of RRALC3 exhibited 99% sequence similarity to Pseudomonas aeruginosa JCM5962. Absence of virulence genes and non-hemolytic activity indicated that RRALC3 is unlikely to be a human pathogen. When the effects of RRALC3 on promotion of plant growth was tested in Pongamia pinnata, it was observed that in Pongamia seedlings treated with a combination of RRALC3 and chemical fertilizer, the dry matter increased by 30.75%. Nitrogen, phosphorus and potassium uptake increased by 34.1%, 27.08%, and 31.84%, respectively, when compared to control. Significant enhancement of total sugar, amino acids and organic acids content, by 23.4%, 29.39%, and 26.53% respectively, was seen in the root exudates of P. pinnata. The carbon content appreciated by 4-fold, when fertilized seedlings were treated with RRALC3. From the logistic equation, the rapid C accumulation time of Pongamia was computed as 43 days longer than the control when a combination of native PGPB and inorganic fertilizer was applied. The rapid accumulation time of N, P and K in Pongamia when treated with the same combination as above was 15, 40 and 33 days longer, respectively, as compared to the control.

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Indigenous plant growth-promoting bacteria enhance plant growth, biomass, and nutrient uptake in degraded forest plants.

Publications

Indigenous plant growth-promoting bacteria enhance plant growth, biomass, and nutrient uptake in degraded forest plants.

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