Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.

ABSTRACT: Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 10⁶ hiPSCs per layer yielded 7.2 × 10⁸ hiPSCs in 4-layer CPs and 1.7 × 10⁹ hiPSCs in 10-layer CPs with pluripotency. hiPSCs were sequentially differentiated into cardiomyocytes (CMs) in a two-dimensional (2D) differentiation protocol. The efficiency of cardiac differentiation using 10-layer CPs with AGV was 66%-87%. Approximately 6.2-7.0 × 10⁸ cells (4-layer) and 1.5-2.8 × 10⁹ cells (10-layer) were obtained with AGV. After metabolic purification w

SUBMITTER: Tohyama S 

PROVIDER: S-EPMC5829307 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature