Ontology highlight
ABSTRACT:
SUBMITTER: Tohyama S
PROVIDER: S-EPMC5829307 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Tohyama Shugo S Fujita Jun J Fujita Chihana C Yamaguchi Miho M Kanaami Sayaka S Ohno Rei R Sakamoto Kazuho K Kodama Masami M Kurokawa Junko J Kanazawa Hideaki H Seki Tomohisa T Kishino Yoshikazu Y Okada Marina M Nakajima Kazuaki K Tanosaki Sho S Someya Shota S Hirano Akinori A Kawaguchi Shinji S Kobayashi Eiji E Fukuda Keiichi K
Stem cell reports 20171005 5
Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 10<sup>6</sup> hiPSCs per layer yielded 7.2 × 10<sup>8</sup> hiPSCs in 4-layer CPs and 1.7 × 10<sup>9</sup> hiPSCs in 10-layer CPs with pluripotency. hiPSCs were sequentially differentiated into cardiomyocy ...[more]