Dataset Information

Single cell polarity in liquid phase facilitates tumour metastasis.

ABSTRACT: Dynamic polarisation of tumour cells is essential for metastasis. While the role of polarisation during dedifferentiation and migration is well established, polarisation of metastasising tumour cells during phases of detachment has not been investigated. Here we identify and characterise a type of polarisation maintained by single cells in liquid phase termed single-cell (sc) polarity and investigate its role during metastasis. We demonstrate that sc polarity is an inherent feature of cells from different tumour entities that is observed in circulating tumour cells in patients. Functionally, we propose that the sc pole is directly involved in early attachment, thereby affecting adhesion, transmigration and metastasis. In vivo, the metastatic capacity of cell lines correlates with the extent of sc polarisation. By manipulating sc polarity regulators and by generic depolarisation, we show that sc polarity prior to migration affects transmigration and metastasis in vitro and in vivo.

SUBMITTER: Lorentzen A

PROVIDER: S-EPMC5830403 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Single cell polarity in liquid phase facilitates tumour metastasis.

Lorentzen Anna A Becker Paul F PF Kosla Jan J Saini Massimo M Weidele Kathrin K Ronchi Paolo P Klein Corinna C Wolf Monika J MJ Geist Felix F Seubert Bastian B Ringelhan Marc M Mihic-Probst Daniela D Esser Knud K Roblek Marko M Kuehne Felix F Bianco Gaia G O'Connor Tracy T Müller Quentin Q Schuck Kathleen K Lange Sebastian S Hartmann Daniel D Spaich Saskia S Groß Olaf O Utikal Jochen J Haferkamp Sebastian S Sprick Martin R MR Damle-Vartak Amruta A Hapfelmeier Alexander A Hüser Norbert N Protzer Ulrike U Trumpp Andreas A Saur Dieter D Vartak Nachiket N Klein Christoph A CA Polzer Bernhard B Borsig Lubor L Heikenwalder Mathias M

Nature communications 20180228 1

Dynamic polarisation of tumour cells is essential for metastasis. While the role of polarisation during dedifferentiation and migration is well established, polarisation of metastasising tumour cells during phases of detachment has not been investigated. Here we identify and characterise a type of polarisation maintained by single cells in liquid phase termed single-cell (sc) polarity and investigate its role during metastasis. We demonstrate that sc polarity is an inherent feature of cells from ...[more]

PMID: 29491397

Similar Datasets

Project description:Cell polarity development is the prerequisite for cell differentiation and generating biodiversity. In the model bacterium Caulobacter crescentus, the polarization of the scaffold protein PopZ during the predivisional cell stage plays a central role in asymmetric cell division. However, our understanding of the spatiotemporal regulation of PopZ localization remains incomplete. In the current study, a direct interaction between PopZ and the new pole scaffold PodJ is revealed, which plays a primary role in triggering the new pole accumulation of PopZ. The coiled-coil 4-6 domain in PodJ is responsible for interacting with PopZ in vitro and promoting PopZ transition from monopolar to bipolar in vivo. Elimination of the PodJ-PopZ interaction impairs the PopZ-mediated chromosome segregation by affecting both the positioning and partitioning of the ParB-parS centromere. Further analyses of PodJ and PopZ from other bacterial species indicate this scaffold-scaffold interaction may represent a widespread strategy for spatiotemporal regulation of cell polarity in bacteria. IMPORTANCE Caulobacter crescentus is a well-established bacterial model to study asymmetric cell division for decades. During cell development, the polarization of scaffold protein PopZ from monopolar to bipolar plays a central role in C. crescentus asymmetric cell division. Nevertheless, the spatiotemporal regulation of PopZ has remained unclear. Here, we demonstrate that the new pole scaffold PodJ functions as a regulator in triggering PopZ bipolarization. The primary regulatory role of PodJ was demonstrated in parallel by comparing it with other known PopZ regulators, such as ZitP and TipN. Physical interaction between PopZ and PodJ ensures the timely accumulation of PopZ at the new cell pole and the inheritance of the polarity axis. Disruption of the PodJ-PopZ interaction impaired PopZ-mediated chromosome segregation and may lead to a decoupling of DNA replication from cell division during the cell cycle. Together, the scaffold-scaffold interaction may provide an underlying infrastructure for cell polarity development and asymmetric cell division.

Dataset Information

Single cell polarity in liquid phase facilitates tumour metastasis.

Publications

Single cell polarity in liquid phase facilitates tumour metastasis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets