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Comparative chemical analysis of volatile compounds of Echinops ilicifolius using hydrodistillation and headspace solid-phase microextraction and the antibacterial activities of its essential oil.

ABSTRACT: The volatile compounds from various parts of Echinops ilicifolius (Compositae) such as flowers, leaves and roots obtained by hydrodistillation (HD) and headspace solid-phase microextraction (HS-SPME) methods were subsequently analysed by GC and GC/MS and compared. Thirty-seven and 20 components of the flower of E. ilicifolius were characterized, representing 99.7% and 100.0% of the total compositions using the HD and HS-SPME methods, respectively. The major constituents of the hydrodistilled oil were identified as linalool (58.6%), geraniol (17.4%), n-dodecane (10.9%) and nerol (5.4%), whereas HS-SPME extract was rich in n-octane (34.0%), n-decane (25.1%), p-cymene (11.1%), ?-terpinene (5.3%) and 1,8-cineole (5.1%). n-Hexadecanoic acid (32.3%), linalool (16.4%) and geraniol (8.3%) were the main components among 43 constituents identified in hydrodistilled extract of the leaf, representing 99.5% of the total components detected, whereas 16 compounds representing 99.9% of the HS-SPME method were identified, among which n-decane (32.6%), p-cymene (14.0%), n-octane (10.3%), limonene (9.2%), ?-terpinene (9.1%), 1,8-cineole (7.9%) and ?-pinene (5.9%) were the major ones. Among 23 components comprising 91.2% of the total hydrodistilled oil detected, n-decane (23.1%), n-dodecane (14.5%), silphiperfol-4,7(14)-diene (11.1%), selin-11-en-4-a-ol (9.5%), n-hexadecanoic acid (7.8%) and n-tetradecane (5.3%) were the main constituents in the root of E. ilicifolius, whereas 1,8-cineole (29.0%), n-decane (12.6%), n-octane (12.6%), camphor (12.0%), p-cymene (9.6%) and ?-terpinene (5.9%) were the main components among the 20 constituents characterized in HS-SPME extract, representing 99.9% of the total components detected. The antibacterial activity of the flower, leaf and root oils of E. ilicifolius against six Gram-positive and Gram-negative bacteria was determined using the MIC method. The growth inhibitory zone (mm) was also measured.

SUBMITTER: Mohebat R

PROVIDER: S-EPMC5830750 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Comparative chemical analysis of volatile compounds of Echinops ilicifolius using hydrodistillation and headspace solid-phase microextraction and the antibacterial activities of its essential oil.

Mohebat Razieh R Bidoki Mina Zare MZ

Royal Society open science 20180214 2

The volatile compounds from various parts of Echinops ilicifolius (Compositae) such as flowers, leaves and roots obtained by hydrodistillation (HD) and headspace solid-phase microextraction (HS-SPME) methods were subsequently analysed by GC and GC/MS and compared. Thirty-seven and 20 components of the flower of E. ilicifolius were characterized, representing 99.7% and 100.0% of the total compositions using the HD and HS-SPME methods, respectively. The major constituents of the hydr ...[more]

PMID: 29515861

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Project description:Volatile organic compounds (VOCs) are byproducts from metabolic pathways that can be detected in exhaled breath and have been reported as biomarkers for different diseases. The gold standard for analysis is gas chromatography-mass spectrometry (GC-MS), which can be coupled with various sampling methods. The current study aims to develop and compare different methods for sampling and preconcentrating VOCs using solid-phase microextraction (SPME). An in-house sampling method, direct-breath SPME (DB-SPME), was developed to directly extract VOCs from breath using a SPME fiber. The method was optimized by exploring different SPME types, the overall exhalation volume, and breath fractionation. DB-SPME was quantitatively compared to two alternative methods involving the collection of breath in a Tedlar bag. In one method, VOCs were directly extracted from the Tedlar bag (Tedlar-SPME) and in the other, the VOCs were cryothermally transferred from the Tedlar bag to a headspace vial (cryotransfer). The methods were verified and quantitatively compared using breath samples (n = 15 for each method respectively) analyzed by GC-MS quadrupole time-of-flight (QTOF) for compounds including but not limited to acetone, isoprene, toluene, limonene, and pinene. The cryotransfer method was the most sensitive, demonstrating the strongest signal for the majority of the VOCs detected in the exhaled breath samples. However, VOCs with low molecular weights, including acetone and isoprene, were detected with the highest sensitivity using the Tedlar-SPME. On the other hand, the DB-SPME was less sensitive, although it was rapid and had the lowest background GC-MS signal. Overall, the three breath-sampling methods can detect a wide variety of VOCs in breath. The cryotransfer method may be optimal when collecting a large number of samples using Tedlar bags, as it allows the long-term storage of VOCs at low temperatures (-80 °C), while Tedlar-SPME may be more effective when targeting relatively small VOCs. The DB-SPME method may be the most efficient when more immediate analyses and results are required.

Dataset Information

Comparative chemical analysis of volatile compounds of Echinops ilicifolius using hydrodistillation and headspace solid-phase microextraction and the antibacterial activities of its essential oil.

Publications

Comparative chemical analysis of volatile compounds of <i>Echinops ilicifolius</i> using hydrodistillation and headspace solid-phase microextraction and the antibacterial activities of its essential oil.

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