Unknown

Dataset Information

0

Cytomegalovirus-specific T-cells are associated with immune senescence, but not with systemic inflammation, in people living with HIV.


ABSTRACT: In people living with HIV (PLWHIV), coinfection with cytomegalovirus (CMV) has been associated with inflammation, immunological ageing, and increased risk of severe non-AIDS related comorbidity. The effect of CMV-specific immune responses on systemic inflammation, immune activation and T-cell senescence was evaluated in 53 PLWHIV treated with combination antiretroviral therapy (cART). Activated-, terminally differentiated-, naïve-, and senescent T-cells were assessed by flow cytometry, and plasma levels of CMV IgG, interleukin-6, tumor necrosis factor-?, high-sensitivity C-reactive protein and soluble-CD14 were measured. In PLWHIV, expression of interleukin-2, tumor necrosis factor-? and interferon-? was measured by intracellular-cytokine-staining after stimulation of T-cells with CMV-pp65, CMV-IE1, and CMV-gB. Increased CMV-specific T-cell responses were associated with a higher ratio of terminally differentiated/naïve CD8+ T-cells and with increased proportions of senescent CD8+ T-cells, but not with systemic inflammation or sCD14. Increased CMV-specific CD4+ T-cell responses were associated with increased proportions of activated CD8+ T-cells. In PLWHIV with expansion of CMV-specific T-cells or increased T-cell senescence, CMV-specific polyfunctionality was maintained. That the magnitude of the CMV-specific T-cell response was associated with a senescent immune phenotype, suggests that a dysregulated immune response against CMV may contribute to the immunological ageing often described in PLWHIV despite stable cART.

SUBMITTER: Ballegaard V 

PROVIDER: S-EPMC5830877 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Cytomegalovirus-specific T-cells are associated with immune senescence, but not with systemic inflammation, in people living with HIV.

Ballegaard Vibe V   Brændstrup Peter P   Pedersen Karin Kaereby KK   Kirkby Nikolai N   Stryhn Anette A   Ryder Lars P LP   Gerstoft Jan J   Nielsen Susanne Dam SD  

Scientific reports 20180228 1


In people living with HIV (PLWHIV), coinfection with cytomegalovirus (CMV) has been associated with inflammation, immunological ageing, and increased risk of severe non-AIDS related comorbidity. The effect of CMV-specific immune responses on systemic inflammation, immune activation and T-cell senescence was evaluated in 53 PLWHIV treated with combination antiretroviral therapy (cART). Activated-, terminally differentiated-, naïve-, and senescent T-cells were assessed by flow cytometry, and plasm  ...[more]

Similar Datasets

| S-EPMC6957152 | biostudies-literature
| S-EPMC6037568 | biostudies-literature
| S-EPMC7140628 | biostudies-literature
| S-EPMC9755486 | biostudies-literature
2024-07-03 | GSE262656 | GEO
2024-02-16 | PXD045155 | Pride
| S-EPMC6718454 | biostudies-literature
| S-EPMC6498051 | biostudies-literature
| S-EPMC9000185 | biostudies-literature
2023-09-13 | GSE224546 | GEO