IGF-1R Promotes Symmetric Self-Renewal and Migration of Alkaline Phosphatase⁺ Germ Stem Cells through HIF-2α-OCT4/CXCR4 Loop under Hypoxia.

ABSTRACT: Hypoxia cooperates with endocrine signaling to maintain the symmetric self-renewal proliferation and migration of embryonic germline stem cells (GSCs). However, the lack of an appropriate in vitro cell model has dramatically hindered the understanding of the mechanism underlying this cooperation. Here, using a serum-free system, we demonstrated that hypoxia significantly induced the GSC mesenchymal transition, increased the expression levels of the pluripotent transcription factor OCT4 and migration-associated proteins (SDF-1, CXCR4, IGF-1, and IGF-1R), and activated the cellular expression and translocalization of the CXCR4-downstream proteins ARP3/pFAK. The underlying mechanism involved significant IGF-1/IGF-1R activation of OCT4/CXCR4 expression through HIF-2α regulation. Picropodophyll

SUBMITTER: Kuo YC 

PROVIDER: S-EPMC5830933 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature